Molecular analysis of carbon monoxide-oxidizing bacteria associated with recent Hawaiian volcanic deposits

Applied and Environmental Microbiology
Kari E Dunfield, G M King

Abstract

Genomic DNA extracts from four sites at Kilauea Volcano were used as templates for PCR amplification of the large subunit (coxL) of aerobic carbon monoxide dehydrogenase. The sites included a 42-year-old tephra deposit, a 108-year-old lava flow, a 212-year-old partially vegetated ash-and-tephra deposit, and an approximately 300-year-old forest. PCR primers amplified coxL sequences from the OMP clade of CO oxidizers, which includes isolates such as Oligotropha carboxidovorans, Mycobacterium tuberculosis, and Pseudomonas thermocarboxydovorans. PCR products were used to create clone libraries that provide the first insights into the diversity and phylogenetic affiliations of CO oxidizers in situ. On the basis of phylogenetic and statistical analyses, clone libraries for each site were distinct. Although some clone sequences were similar to coxL sequences from known organisms, many sequences appeared to represent phylogenetic lineages not previously known to harbor CO oxidizers. On the basis of average nucleotide diversity and average pairwise difference, a forested site supported the most diverse CO-oxidizing populations, while an 1894 lava flow supported the least diverse populations. Neither parameter correlated with previous es...Continue Reading

References

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Citations

May 18, 2011·Microbial Ecology·Laura C KellyXavier LeRoux
Jan 17, 2007·Nature Reviews. Microbiology·Gary M King, Carolyn F Weber
Dec 20, 2013·Environmental Technology·Sean NguyenSonia M Tiquia-Arashiro
Jul 22, 2008·Applied and Environmental Microbiology·Jason M Smith, Andrew Ogram
Feb 9, 2010·Applied and Environmental Microbiology·C F Weber, G M King
Dec 8, 2005·Applied and Environmental Microbiology·J Tolli, G M King
Apr 7, 2006·Applied and Environmental Microbiology·Patrick D Schloss, Jo Handelsman
May 25, 2006·Annual Review of Microbiology·Irene Wagner-Döbler, Hanno Biebl
Mar 28, 2008·Environmental Microbiology·Cristina Takacs-VesbachAnna-Louise Reysenbach
Mar 7, 2014·Environmental Microbiology Reports·Marcela HernándezRalf Conrad
Apr 16, 2015·Microbial Biotechnology·Ying GuoXinshu Zhuang
Aug 1, 2015·FEMS Microbiology Ecology·Yasuko YonedaYoshihiko Sako
Apr 26, 2008·FEMS Microbiology Reviews·Catherine A Lozupone, Rob Knight
Mar 27, 2007·FEMS Microbiology Ecology·Vicente Gomez-AlvarezKlaus Nüsslein
Oct 25, 2006·FEMS Microbiology Ecology·Gary M King
Oct 14, 2016·Microbial Ecology·Dorsaf KerfahiJonathan M Adams
May 29, 2020·FEMS Microbiology Ecology·Corinne Biderre-PetitRaphaël Paris

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