Molecular analysis of crossing-over in the CMH in two Tunisian families with aplastic bone marrow

Pathologie-biologie
N Mahfoudh LahianiH Makni

Abstract

In order to select compatible human leucocytes antigens (HLA) donors for bone marrow graft, all the members of 76 families were typed by serology for HLA class I (A and B locus) and class II (DR, DQ locus) by polymerase chain-reaction-sequence-specific primes (PCR-SSP). The HLA typing interpretation revealed the existence of crossing-over in major histocompatibility (CMH) regions for two families, AB and AT, with aplastic bone marrow. The study of crossing-over site has needed the genotyping of seven short tandem repeat (STR) markers located on the short arm of chromosome 6 (D6S291, D6S273, TNFa, C1.2.C, C3.2.11, D6S265, D6S276), using ABI Prism 310 sequencer. HLA and STR Haplotypic analysis enabled us to confirm the crossing-over between locus B and DR in AB family and between locus A and B in AT family. Based in this study, we recommend to be careful in the interpretation of the results of HLA typing between donors and recipients of bone marrow. Complementary investigations should be accomplished for studying genetic abnormalities, which would be involved in this pathology.

References

Feb 1, 1997·Human Pathology·P S DickmanJ Blatt
Mar 1, 1997·Tissue Antigens·A FoissacA Cambon-Thomsen
Jan 23, 1998·Human Immunology·K A SullivanM Bryer-Ash
May 13, 1999·Immunological Reviews·M Carrington

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Citations

Jun 18, 2014·Expert Opinion on Drug Safety·Mona KargarKheirollah Gholami

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