PMID: 11311186Apr 20, 2001Paper

Molecular analysis of DHFR and DHPS genes in P. falciparum clinical isolates from the Haut--Ogooué region in Gabon

Acta Tropica
D P Mawili-MboumbaF Ntoumi

Abstract

The main objective of this work was to determine the prevalence of mutations in genes coding for the dihydropteroate synthase (DHPS) and the dihydrofolate reductase (DHFR) enzymes which are implicated in resistance of P. falciparum to antifolate (pyrimethamine-sulfadoxine (P/S)). In this study, 117 human blood samples were collected at Franceville located in the region of Haut-Ogooué (South-eastern Gabon). In this area, a relatively low level of sensitivity of Plasmodium falciparum to P/S has been reported with 18.2% of RII and 12.1% of RI resistance. A nested polymerase chain reaction was used to amplify a fragment of the DHFR gene containing codon 108, where a point mutation causing a Serine (wild type) to Asparagine or to a Threonine (resistant types) change occurs in pyrimethamine resistant parasites. Eleven DHFR fragments were sequenced and mutations occurring at codons 51, 59 and 108 were analysed. The DHPS gene was amplified by polymerase chain reaction (PCR) and sequenced directly or after cloning. Variant amino acid residues 436, 437, 540, 581, 613 associated with sulfadoxine resistance were analysed. The analysis of codon 108 of the DHFR gene was undertaken for 81 isolates. More than one DHFR P. falciparum genotype wa...Continue Reading

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Citations

Apr 9, 2002·The Lancet Infectious Diseases·Chansuda WongsrichanalaiSteven R Meshnick
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sungano MharakurwaPeter Agre
Jun 19, 2019·Malaria Research and Treatment·Jeanne Vanessa Koumba LengongoDenise Patricia Mawili-Mboumba
Mar 29, 2021·Journal of Global Antimicrobial Resistance·Fleuramie Mirembou BoukoumbaSandrine Lydie Oyegue-Liabagui

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