Molecular analysis of genetically determined target organ abnormalities in spontaneous autoimmune thyroiditis

Immunobiology
G WickI Lefkovits

Abstract

We have shown in earlier studies, that the development of spontaneous autoimmune thyroiditis (SAT) in chickens of the Obese strain (OS) depends on the presence of both, two dominant genes coding for an altered immune regulation and one recessive gene responsible for the susceptibility of the target organ for the autoimmune attack. The product(s) of the latter is (are) still not known. The present study was aimed at identifying possible candidates of cellular components of the thyroid gland of OS chicken and its SAT susceptible parental Cornell C-strain (CS) by high resolution 2-dimensional (2D) gel electrophoresis. For this purpose organ cultures of the thyroid, bursa, thymus and liver were established and the synthesized polypeptides were labelled by 35S-methionine. OS and CS organs were compared with those of healthy normal White Leghorn (NWL) controls. The autoradiographs of the 2D-gels obtained from individual samples after various labelling periods were subjected to comparative analysis. We have found both quantitative and qualitative differences of polypeptide spots between OS/CS and NWL organ samples, some of them specific for the thyroid gland. Although one has to be aware that in this multidimensional analytical approa...Continue Reading

References

Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Jan 1, 1989·Immunologic Research·R S Sundick
Aug 1, 1989·Clinical Immunology and Immunopathology·G KroemerG Wick
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I LefkovitsJ Kettman
Jul 1, 1984·Clinical Immunology and Immunopathology·G AichingerG Wick

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Citations

Jan 1, 1993·Thyroid : Official Journal of the American Thyroid Association·R Volpé
Oct 17, 1998·DNA Sequence : the Journal of DNA Sequencing and Mapping·M SheppardM Johnson
Mar 1, 1995·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·R Volpé

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