Molecular analysis of Giardia duodenalis isolates from symptomatic and asymptomatic children from La Habana, Cuba

Parasite Epidemiology and Control
Luis Enrique Jerez PueblaNorbert Müller

Abstract

Giardiasis is considered the most common intestinal parasitic disease in humans worldwide. In Cuba, this infection has particularly a strong clinical impact on the child population. Giardia duodenalis is a highly diverse protozoan, which comprises a complex of eight morphologically identical genetic assemblages, further divided into sub-assemblages. The present study used triose phosphate isomerase (tpi) and small-subunit ribosomal RNA (SSU rRNA) genes as genetic markers for the identification of G. duodenalis assemblages and sub-assemblages in correlation with clinical and epidemiological data in children attended at the Paediatric Hospital "William Soler" and at Pedro Kouri Institute, between 2015 and 2016. A prevalence of 8% of G. duodenalis infection was recorded in stool samples after concentration techniques from 68 children out of 847 analysed. A 100% detection of Giardia DNA was achieved by a SSU-rRNA PCR, whereas DNA from 63 of 68 (92.6%) was successfully amplified by tpi-PCR. By this assemblage-specific tpi-PCR 32 (50.8%) assemblage B, 17 (27.0%) assemblage A and 14 (22.2%) mixed infection (A + B) were identified. Assemblage B was significantly (P < 0.02) more frequently found in children with diarrhoea. Sequence anal...Continue Reading

Citations

Jun 9, 2019·Animals : an Open Access Journal From MDPI·Elsa M Godínez-GalazGerminal J Cantó-Alarcón
Apr 3, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Cláudia Rosana Trevisani CorrêaSemíramis Guimarães
Sep 22, 2018·Parasites & Vectors·Xun MaGuang-Hui Zhao
Oct 18, 2020·Trends in Parasitology·Paul CapewellWilliam Weir
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·K MravcováM Goldová

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Methods Mentioned

BETA
genotyping
PCR
PCRs
electrophoresis

Software Mentioned

Molecular Evolutionary Genetics Analysis
Epi
MEGA
Info
BioEdit
ClustalW
EPIDAT
MEGA6

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