PMID: 7528877Jan 1, 1995Paper

Molecular analysis of hypoxanthine phosphoribosyltransferase gene deletions induced by alpha- and X-radiation in human lymphoblastoid cells

Mutation Research
C Y BaoW D Sedwick

Abstract

Mutations caused by exposure to X-radiation and to radon and its decay products were compared in the hprt gene of a human lymphoblastoid cell line. Thirty-one X-radiation-induced, 29 radon-induced, and 24 spontaneous mutants were recovered from cell cultures under identical conditions except for the exposure to radiation. Seven spontaneous point mutations were recovered and DNA sequenced. These mutations included three C:G-->T:A transitions. These spontaneous point mutations were located in the exon or splice donor regions of five of the nine hprt exons. Four X-radiation-induced and three radon-induced point mutations were also analyzed by DNA sequencing. The frequency of induced mutants at the D0 doses for radon and X-radiation respectively were 5 x 10(-6) and 4.5 x 10(-6). Deletions were the predominant mutations recovered from both radon- and X-irradiated cells. Eighty-one percent of the mutants from X-radiation-treated cultures, 86% of the radon-treated cultures, and 63% of the spontaneous mutants involved deletions. Deletions involving exon and intron DNA, as well as intron DNA alone, were found to inactivate the hprt gene and result in a selectable HPRT- phenotype. Among the deletion mutants, however, only 21% of the spon...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C WaldrenT T Puck
Jan 1, 1992·Somatic Cell and Molecular Genetics·C Y LiJ B Little
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E A HendricksonD T Weaver
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R D LittleD Schlessinger
Jan 1, 1991·Environmental and Molecular Mutagenesis·J A NicklasR J Albertini
Apr 1, 1990·Genomics·A EdwardsW Ansorge
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C L Vnencak-JonesP H Seeburg
Aug 1, 1986·Mutation Research·A W SkulimowskiM Haliandros
Nov 1, 1986·Somatic Cell and Molecular Genetics·G UrlaubJ Hamlin
Mar 1, 1988·Mutation Research·M JanatipourA A Morley
Sep 1, 1988·Mutation Research·I N Gennett, W G Thilly
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P KavathasR DeMars
Jan 1, 1993·Environmental and Molecular Mutagenesis·C R GiverA J Grosovsky

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Citations

Nov 10, 1998·International Journal of Cancer. Journal International Du Cancer·N SuzukiY Hayashi
Oct 1, 1995·Mutation Research·H J MartusJ Vijg
Jul 27, 2001·Mutation Research·G K KuipersM V Lafleur
Sep 1, 1996·Mutation Research·J R WilliamsM H Mabry
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W ThomasT Magnuson
Aug 3, 2012·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Jian TongYan An
Oct 30, 1998·Genes & Genetic Systems·N ShikazonoS Tano
May 1, 1996·Environmental Health Perspectives·A W HsieJ L Schwartz
Jul 25, 2013·International Journal of Molecular Sciences·Aaron RobertsonAlison Curnow
Aug 1, 1996·International Journal of Radiation Biology·S A AmundsonR T Okinaka
May 12, 2005·Environmental and Molecular Mutagenesis·Patrick O'NeillRichard Albertini
Jan 1, 1997·Environmental and Molecular Mutagenesis·A D da Cruz, B W Glickman
Feb 11, 2000·Radiation Research·J L SchwartzA W Hsieb
May 11, 2000·Journal of Radiation Research·J H Hendry
May 23, 2008·Radiation Research·Kai RothkammMarkus Löbrich

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