Molecular analysis of lipooligosaccharide biosynthesis in Neisseria gonorrhoeae.

Infection and Immunity
E F Petricoin, Daniel C Stein

Abstract

A HindIII gene bank of Neisseria gonorrhoeae MUG116 was constructed in the cosmid vector pHC79. A cosmid (pSY81) was isolated that was able to convert N. gonorrhoeae FA5100 to reactivity with monoclonal antibody (MAb) 2-1-L8. Several MAb-reactive transformants were isolated and characterized with respect to lipooligosaccharide (LOS) production as analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, their ability to react with two other LOS-specific MAbs (3F11 and O6B4), and Southern blot analysis. Escherichia coli containing the clone had altered lipopolysaccharide expression as determined by electrophoretic analysis; however, no reactivity was seen with gonococcus-specific MAbs. The introduction of pSY81 into FA5100 had a pleiomorphic effect, giving rise to transformants having the full parental phenotype or transformants lacking reactivity to a combination of LOS-specific MAbs. Southern blot analysis indicated that the LOS biosynthetic mutation in FA5100 was not due to chromosomal rearrangement or large deletions.

References

Aug 1, 1976·Antimicrobial Agents and Chemotherapy·S A MorseB H Iglewski
Feb 1, 1986·The Journal of Infectious Diseases·P F SparlingM So
Oct 1, 1986·Infection and Immunity·R MandrellJ M Griffiss
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B W GibsonJ M Griffiss
May 1, 1986·Journal of Bacteriology·A RehemtullaK E Sanderson
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Feb 1, 1984·The Journal of Infectious Diseases·W M ShaferP F Sparling
Jul 18, 1984·Biochemical and Biophysical Research Communications·P Chomczynski, P K Qasba

❮ Previous
Next ❯

Citations

Jan 1, 1995·Molecular Microbiology·E T SchwanJ P van Putten
Feb 24, 2001·Journal of Bacteriology·D ArkingD C Stein
May 1, 1993·Molecular Microbiology·B D RobertsonJ P van Putten
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·A BanerjeeD C Stein
Nov 1, 1990·Biomedical & Environmental Mass Spectrometry·N J PhillipsJ M Griffiss
Jan 15, 1999·Critical Reviews in Microbiology·C M Kahler, D S Stephens
Nov 1, 1994·Journal of Bacteriology·R C SandlinD C Stein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.