Molecular analysis of maltotriose active transport and fermentation by Saccharomyces cerevisiae reveals a determinant role for the AGT1 permease.

Applied and Environmental Microbiology
Sergio L AlvesBoris U Stambuk

Abstract

Incomplete and/or sluggish maltotriose fermentation causes both quality and economic problems in the ale-brewing industry. Although it has been proposed previously that the sugar uptake must be responsible for these undesirable phenotypes, there have been conflicting reports on whether all the known alpha-glucoside transporters in Saccharomyces cerevisiae (MALx1, AGT1, and MPH2 and MPH3 transporters) allow efficient maltotriose utilization by yeast cells. We characterized the kinetics of yeast cell growth, sugar consumption, and ethanol production during maltose or maltotriose utilization by several S. cerevisiae yeast strains (both MAL constitutive and MAL inducible) and by their isogenic counterparts with specific deletions of the AGT1 gene. Our results clearly showed that yeast strains carrying functional permeases encoded by the MAL21, MAL31, and/or MAL41 gene in their plasma membranes were unable to utilize maltotriose. While both high- and low-affinity transport activities were responsible for maltose uptake from the medium, in the case of maltotriose, the only low-affinity (K(m), 36 +/- 2 mM) transport activity was mediated by the AGT1 permease. In conclusion, the AGT1 transporter is required for efficient maltotriose fe...Continue Reading

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Citations

Jul 3, 2009·Applied Microbiology and Biotechnology·Akinori MatsushikaShigeki Sawayama
Mar 5, 2013·Current Genetics·J Horák
Feb 3, 2009·Applied and Environmental Microbiology·Virve VidgrenJohn Londesborough
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Tatsuya FujiiShigeki Sawayama
May 9, 2012·Molecular Genetics and Genomics : MGG·Farbod BabrzadehBoris U Stambuk
May 6, 2015·Molecular Genetics and Genomics : MGG·Dianguang XiongChengming Tian
Apr 21, 2010·FEMS Yeast Research·Virve VidgrenJohn Londesborough
Jul 12, 2018·Letters in Applied Microbiology·S L AlvesB U Stambuk
Aug 3, 2014·Eukaryotic Cell·Jürgen Wendland
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Véronique Leh LouisJean-Luc Souciet
Jan 18, 2020·Annual Review of Food Science and Technology·B GibsonV Vidgren
Apr 20, 2019·Frontiers in Genetics·Arthur R Gorter de VriesJean-Marc G Daran
Jul 28, 2019·Applied Microbiology and Biotechnology·Kristoffer KrogerusBrian Gibson
Mar 15, 2020·Applied Microbiology and Biotechnology·Kristoffer Krogerus, Brian Gibson
Apr 25, 2020·Archives of Microbiology·Évelyn T BarrilliSérgio L Alves
Apr 18, 2018·Antonie van Leeuwenhoek·Thalita Macedo AraújoRogelio Lopes Brandão
Apr 10, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ane Catarine Tosi CostaPatricia M B Fernandes

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