Molecular analysis of microbial communities identified in different developmental stages of Ixodes scapularis ticks from Westchester and Dutchess Counties, New York

Environmental Microbiology
Claudia X MorenoFelipe C Cabello

Abstract

Ixodes scapularis ticks play an important role in the transmission of a wide variety of pathogens between various mammalian species, including humans. Pathogens transmitted by ticks include Borrelia, Anaplasma and Babesia. Although ticks may harbour both pathogenic and non-pathogenic microflora, little is known about how the diversity of the microflora within ticks may influence the transmission of pathogens. To begin addressing this question, we examined the composition of bacterial communities present in Ixodes scapularis collected from Westchester and Dutchess Counties, New York State, at different developmental and nutritional stages. Genetic fingerprints of bacterial populations were generated by temporal temperature gradient gel electrophoresis (TTGE) separation of individual polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, followed by DNA sequence analysis for bacterial identification. The fingerprints of the TTGE bands were grouped into five clusters. The most abundant DNA sequence found in all the samples was Rickettsia, followed by Pseudomonas and Borrelia. Ralstonia, Anaplasma, Enterobacterias, Moraxella, Rhodococcus and uncultured proteobacterium were present as well. We also determined the prevale...Continue Reading

References

Sep 1, 1991·Journal of Medical Entomology·J F LevineR T Turner
Oct 1, 1995·The American Journal of Tropical Medicine and Hygiene·A M De Silva, E Fikrig
Oct 1, 1996·Medical and Veterinary Entomology·J N MakumiM Baylis
Mar 1, 1997·The American Journal of Tropical Medicine and Hygiene·I SchwartzD Fish
Mar 21, 1998·Emerging Infectious Diseases·S VardeI Schwartz
Jun 29, 1999·Current Opinion in Microbiology·G Muyzer
Jan 3, 2001·Applied and Environmental Microbiology·A G SpeksnijderH J Laanbroek
Jul 14, 2001·The New England Journal of Medicine·S J De MartinoE Fikrig
Jul 31, 2002·Applied and Environmental Microbiology·Jean-Claude OgierAgnes Delacroix-Buchet
Oct 25, 2002·Proceedings. Biological Sciences·Steven van BormJohan Billen
Dec 3, 2002·Journal of Microbiological Methods·Claudia Schabereiter-GurtnerSabine Rölleke
Dec 4, 2002·Environmental Microbiology·N FrominP Rossi
Dec 27, 2002·The Journal of General and Applied Microbiology·Jorge W. Santo Domingo
Feb 27, 2003·Emerging Infectious Diseases·John G GerrardNeil Fraser
Mar 18, 2004·Microbes and Infection·Thierry De MeeûsFrançois Renaud
Jun 19, 2004·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·J Süss, C Schrader
Jul 10, 2004·Journal of Clinical Microbiology·Joshua W CourtneyRobert F Massung
Mar 11, 2005·Emerging Infectious Diseases·Sarah J KogutDennis J White
Mar 31, 2005·The Japanese Journal of Physiology·Tomoko NakamotoHidehiko Komine

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Citations

Sep 1, 2009·International Health·Aurélie RenvoiséDidier Raoult
Mar 19, 2011·Vector Borne and Zoonotic Diseases·Jimmy Ninh PhanJianmin Zhong
Apr 3, 2012·Applied and Environmental Microbiology·Itai LalzarYuval Gottlieb
Sep 6, 2008·Canadian Journal of Microbiology·Robert I GrahamChristopher J Lucarotti
Mar 6, 2010·Emerging Infectious Diseases·Sam R Telford, Gary P Wormser
Jan 8, 2015·International Journal of Systematic and Evolutionary Microbiology·Timothy J KurttiUlrike G Munderloh
Apr 15, 2015·Ticks and Tick-borne Diseases·Maria OgrzewalskaMarcelo B Labruna
Aug 21, 2015·PeerJ·Alyssa D AmmazzalorsoSergios-Orestis Kolokotronis
May 6, 2015·Trends in Parasitology·Sukanya Narasimhan, Erol Fikrig
Oct 2, 2008·Journal of Medical Entomology·Cornelia SilaghiKurt Pfister
Sep 25, 2016·Microbiological Research·Lina Marcela Silva-BedoyaClaudia Ximena Moreno-Herrera
Sep 3, 2016·Molecular Ecology·Christine P ZolnikSergios-Orestis Kolokotronis
Jul 8, 2015·Applied and Environmental Microbiology·Will Van TreurenSteven R Meshnick
Oct 5, 2013·Clinical Microbiology Reviews·Philippe ParolaDidier Raoult
Mar 22, 2008·FEMS Microbiology Ecology·Leo van OverbeekWillem Takken
Nov 2, 2018·Proceedings. Biological Sciences·Dolores GennéMaarten J Voordouw
Nov 7, 2017·Frontiers in Veterinary Science·Andrea S Varela-StokesSteven C Ricke
May 28, 2020·Frontiers in Cellular and Infection Microbiology·Melina Garcia GuizzoLudek Zurek
May 16, 2020·PloS One·R Jory BrinkerhoffWayne L Hynes
Jun 24, 2017·Frontiers in Cellular and Infection Microbiology·Sarah I BonnetOlivier Duron
Jun 28, 2018·The ISME Journal·Benjamin D RossJoseph D Mougous

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