Molecular analysis of mxbD and mxbM, a putative sensor-regulator pair required for oxidation of methanol in Methylobacterium extorquens AM1.

Microbiology
Amy L SpringerMary E Lidstrom

Abstract

Five genes are thought to be required for transcription of methanol oxidation genes in Methylobacterium strains. These putative regulatory genes include mxcQE, which encode a putative sensor-regulator pair, and mxbDM and mxaB, whose functions are less well-understood. In this study, mxbDM in Methylobacterium extorquens AM1 were shown to be required for expression of a xylE transcriptional fusion to the structural gene for the large subunit of methanol dehydrogenase (mxaF), confirming the role of these genes in transcriptional regulation of mxaF. The nucleotide sequence suggests that mxbD encodes a histidine protein kinase with two transmembrane domains and that mxbM encodes a DNA-binding response regulator. A xylE transcriptional fusion to the putative mxbD promoter showed low-level expression in wild-type cells grown on one-carbon (C1) compounds and no detectable expression in cells grown on succinate. Deletion analysis of this promoter construct showed that the region 229-129 bp upstream of the start of mxbD is required for expression. The expression of the mxbD-xylE fusion was examined in each of the five known regulatory mutant classes. xylE expression was reduced to non-detectable levels in MxcQ and MxcE mutants, but was n...Continue Reading

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Citations

Aug 30, 2011·Journal of Bacteriology·Elizabeth SkovranMary E Lidstrom
May 6, 2003·Journal of Bacteriology·Ludmila ChistoserdovaMary E Lidstrom
May 13, 2014·Applied Microbiology and Biotechnology·Jan T KeltjensHuub J M Op den Camp
Nov 30, 2014·Applied Microbiology and Biotechnology·Andrea M OchsnerJulia A Vorholt
Jun 29, 2017·Molecular Plant-microbe Interactions : MPMI·Andrea KrauseBarbara Reinhold-Hurek
Apr 21, 2019·Applied and Environmental Microbiology·Abhishek Anil Dubey, Vikas Jain
Jan 29, 2011·Applied Biochemistry and Biotechnology·Shanmugam JayashreeSundaram Seshadri
Jan 18, 2019·Molecular Microbiology·Andrea M OchsnerJulia A Vorholt

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