Molecular analysis of neutralizing epitopes on outer surface proteins A and B of Borrelia burgdorferi.

Infection and Immunity
J MaR T Coughlin

Abstract

The neutralizing epitopes of the major outer surface proteins A and B (OspA and OspB) of Borrelia burgdorferi B31 were investigated by epitope mapping using overlapping synthetic peptides, encompassing full-length OspA and OspB, and antiborrelial monoclonal antibodies (MAbs). OspA MAb N4B12 and OspB MAbs N5G5, W7C2, and P4D1 displayed a complement-independent antiborrelial activity, and complement failed to enhance the antiborrelial activity, as measured by a sensitive colorimetric assay. A combination of N4B12 with N5G5 displayed a higher antiborrelial activity than did the MAbs individually. OspA MAbs B3G11 and L3B5, however, exhibited a significant antiborrelial activity only in the presence of complement. Epitope mapping showed that B3G11 bound to one OspA synthetic peptide with the sequence of amino acids 247 to 256 (QYDSNGTKLE) and produced more than sixfold-higher reactivity than with other sequences, as measured by an enzyme-linked immunosorbent assay. OspB MAb N5G5 bound to an OspB peptide with the sequence of amino acids 211 to 220 (TLKREIEKDG), yielding at least threefold-higher reactivity than with other sequences. These two peptide sequences were found to contain neutralizing epitopes. Other MAbs had weak binding a...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1992·International Journal of Systematic Bacteriology·G BarantonP A Grimont
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E FikrigR A Flavell
Feb 1, 1991·The Journal of Infectious Diseases·H FahrerA Aeschlimann
Mar 1, 1991·The Journal of Infectious Diseases·C S PaviaS Levine
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·U E SchaibleM M Simon
Aug 31, 1989·The New England Journal of Medicine·A C Steere
Sep 1, 1989·Reviews of Infectious Diseases·B J LuftR J Dattwyler
Jan 19, 1989·The New England Journal of Medicine·C C LastavicaR D Deblinger
Sep 1, 1986·Journal of the American Academy of Dermatology·B W Berger
Jun 18, 1982·Science·W BurgdorferJ P Davis
Jan 1, 1993·The Journal of Infectious Diseases·A SadzieneA G Barbour
Jun 11, 1993·Science·A G Barbour, D Fish

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Citations

Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S E Connolly, J L Benach
Jan 13, 2004·Clinical and Diagnostic Laboratory Immunology·Erik L MunsonRonald F Schell
Jan 24, 2006·Clinical and Vaccine Immunology : CVI·Erik L MunsonRonald F Schell
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susan MalkielJorge L Benach
Jul 23, 2003·Infection and Immunity·Matthew B LawrenzSteven J Norris
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L I KatonaJ L Benach
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sean E ConnollyJorge L Benach
Jul 3, 2002·Clinical and Diagnostic Laboratory Immunology·Steven M CallisterJennifer A Marks
Feb 24, 2004·Infection and Immunity·Erol FikrigRichard A Flavell
Feb 17, 2005·The Journal of Biological Chemistry·Michael BeckerCatherine L Lawson
May 3, 2005·Nature Reviews. Microbiology·Sean E Connolly, Jorge L Benach
Feb 6, 2020·Antimicrobial Agents and Chemotherapy·Gabrielle RichardGreg Hussack
Oct 11, 2003·FEMS Microbiology Reviews·Juan AnguitaErol Fikrig
Jul 1, 1996·Clinical and Diagnostic Laboratory Immunology·S M CallisterS D Lovrich

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