Molecular analysis of P16(Ink4)/CDKN2 and P15(INK4B)/MTS2 genes in primary human testicular germ cell tumors

The Journal of Urology
A HeidenreichS Srivastava

Abstract

p16 (MTS 1) and p15 (MTS 2) are negative regulators of cell cycle progression at the G1 cell cycle checkpoint and function as tumor suppressor genes (TSG). Both p15 and p16 are located on chromosome 9p21 and alterations have been demonstrated in a variety of human malignancies and human cancer cell lines. In testicular germ cell tumors (TGCT) loss of heterozygosity of 9p21 to 23 has been observed in 41% to 72% of informative cases. The aim of our study was to examine TGCT and testicular cancer cell lines for deletions and mutations of the p15 and p16 genes. 24 testicular germ cell tumors, 4 testicular cancer cell lines (TERA I, TERA II, HTE, HTH), 8 benign testicular tumors and 9 samples of normal testicular parenchyma were examined. Examinations for loss of heterozygosity (LOH) on 9p21 were performed for IFNA, D9S171, D9S126, D9S161, D9S1748 and PKY9 locus using Southern Blot analysis. Southern Blot analysis of the p16 gene to examine gross alterations was done using random primer labeled p16 cDNA. In addition all samples were examined for mutations of exons 1 to 3 of p16 and exons 1 to 2 of p15 by polymerase chain reaction (PCR) followed by single strand conformation polymorphism (SSCP) analysis on MDE-gels. All observed band...Continue Reading

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Citations

Oct 19, 2002·Urology·George M YousefEleftherios P Diamandis
May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Rolf I Skotheim, Ragnhild A Lothe
May 23, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jirina BartkovaJiri Bartek
Jun 7, 2000·Current Opinion in Oncology·R T Oliver
Jun 3, 2004·Cancer Genetics and Cytogenetics·Finn Edler von Eyben
Apr 13, 2006·Acta Oto-laryngologica·G Lehnerdt, M Fischer
Feb 6, 2003·Cancer·Martijn F Lutke HolzikHarald J Hoekstra
May 20, 2003·The Journal of Pathology·Jirina BartkovaJiri Bartek
May 20, 2003·The Journal of Pathology·Diana C J SpieringsSteven de Jong
Nov 17, 2012·The American Journal of Surgical Pathology·Jennifer B KumLiang Cheng
Jun 20, 2002·Journal of Neuropathology and Experimental Neurology·Yoshifumi OkadaDavid N Louis
Jan 6, 2001·Scandinavian Journal of Urology and Nephrology. Supplementum·M RørthN E Skakkebaek

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