Molecular analysis of products of conception obtained by hysteroembryoscopy from infertile couples

Journal of Assisted Reproduction and Genetics
Inmaculada Campos-GalindoCarmen Rubio

Abstract

To analyze the molecular cytogenetic data obtained from products of conception (POC) obtained by selective biopsy of first trimester miscarriages and to estimate the rate of chromosomal anomalies in miscarriages from pregnancies achieved by natural conception (NC) or by assisted reproductive technology (ART) interventions. We used KaryoLite™ BoBs™ (PerkinElmer LAS, Wallac, Turku, Finland) technology to analyze 189 samples from ART or NC pregnancies. All POC were successfully evaluated. A higher incidence of chromosomal abnormalities was observed in POC after ART using the patient's own oocytes than from NC pregnancies (62.7% vs. 40.6%; p < 0.05). The lowest incidence of chromosomal abnormalities was observed in POCs ART using donor eggs from women younger than 35 years (12.8%). No statistical differences in the percentage of abnormal miscarriages were observed in correlation with sperm concentration: a sperm concentration less than 5 million/mL produced 75% abnormal results and a concentration higher than 5 million/mL produced 51%. POC analysis is essential to determine the cause of pregnancy loss. Using culture-independent molecular biology techniques to analyze POCs avoids limitations such as growth failure and reduces the ti...Continue Reading

References

Sep 15, 1990·Lancet·G M Stirrat
Jan 1, 1983·Annals of Human Genetics·T HassoldJ A Yamane
Oct 1, 1980·Annals of Human Genetics·T HassoldP A Jacobs
Jan 1, 1980·Human Genetics·T KajiiS Avirachan
Jan 24, 1998·Human Genetics·K S Reddy
Nov 11, 2003·Fertility and Sterility·Jaime FerroVicente Serra
Jun 24, 2004·The Journal of Obstetrics and Gynaecology Research·Masaji NagaishiJudith Knops
Jun 25, 2004·American Journal of Reproductive Immunology : AJRI·Rushdia Z Yusuf, Rizwan Naeem
Mar 8, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joris R VermeeschKoen Devriendt
Apr 19, 2005·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Joshua MenashaNataline B Kardon
Aug 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sherry A Dunbar
Nov 15, 2006·American Journal of Medical Genetics. Part a·Blake C BallifBassem A Bejjani
Feb 17, 2007·Clinical Obstetrics and Gynecology·Mary Stephenson, William Kutteh
Jul 4, 2007·American Journal of Medical Genetics. Part a·Sau W CheungV Reid Sutton
Jul 21, 2009·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Caroline RobberechtJoris Robert Vermeesch
Aug 1, 2009·Fertility and Sterility·Kerri MarquardRuth B Lathi
Aug 3, 2010·Journal of Assisted Reproduction and Genetics·Sofia DóriaFilipa Carvalho
Nov 26, 2010·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Melody C CaraminsMichael F Buckley
Apr 3, 2012·Prenatal Diagnosis·François VialardFrancesca R Grati
Apr 24, 2012·Fertility and Sterility·Nasser Al-AsmarCarmen Rubio
Jul 28, 2012·Fertility and Sterility·UNKNOWN Practice Committee of the American Society for Reproductive Medicine
Nov 22, 2012·Prenatal Diagnosis·Francesca R GratiFrançois Vialard

❮ Previous
Next ❯

Citations

Aug 25, 2018·Molecular Cytogenetics·Xiangyu ZhuYali Hu
May 6, 2021·Journal of Minimally Invasive Gynecology·Shiri WeinbergOshri Barel
Dec 24, 2020·Genes·Sandra Garcia-HerreroJavier Garcia-Planells

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.