PMID: 9529069Apr 7, 1998Paper

Molecular analysis of Shiga toxigenic Escherichia coli O111:H- proteins which react with sera from patients with hemolytic-uremic syndrome

Infection and Immunity
E VossJames C Paton

Abstract

Western blot analysis was used to assess the reactivity of convalescent-phase sera from patients who were associated with an outbreak of hemolytic-uremic syndrome (HUS) caused by fermented sausage contaminated with Shiga toxin-producing Escherichia coli (STEC). The predominant STEC isolated from HUS patients belonged to serotype O111:H-, and reactivity to O111:H- whole-cell lysates, treated or untreated with proteinase K, was examined. As expected, all five serum samples demonstrated a marked anti-lipopolysaccharide response, but several protein bands were also immunoreactive, particularly one with an apparent size of 94 kDa. One convalescent-phase serum sample was subsequently used to screen an O111:H- cosmid bank and 2 of 900 cosmid clones were found to be positive, both of which contained a similar DNA insert. Western blot analysis of one of these clones identified three major immunoreactive protein bands of approximately 94, 70, and 50 kDa. An immune response to the three proteins was detectable with all five convalescent-phase serum samples but not with healthy human serum. Immunoreactive 94- and 50-kDa species were produced by a deletion derivative of the cosmid containing a 7-kb STEC DNA insert. Sequence analysis of this...Continue Reading

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Citations

Sep 24, 1999·Current Opinion in Nephrology and Hypertension·S P Andreoli
Aug 16, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Johanna ScheiringLothar Bernd Zimmerhackl
Aug 26, 2000·Scandinavian Journal of Infectious Diseases·P N Goldwater, K A Bettelheim
Dec 26, 2001·Journal of Bacteriology·Cheryl L Tarr, Thomas S Whittam
Nov 7, 2006·Scandinavian Journal of Immunology·L B Zapata-QuintanillaS B Carbonare
Nov 28, 2002·Journal of Clinical Microbiology·W L ZhangH Schmidt

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