Molecular Analysis of Single Tumor Glands Using the Crypt Isolation Method in Endometrial Carcinomas

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Takayuki NagasawaToru Sugiyama

Abstract

Endometrial adenocarcinomas are characterized by the presence of many single tumor glands in which multiple genetic changes have accumulated. To elucidate the differences in molecular abnormalities among single tumor glands, individual tumor glands were analyzed and microsatellite alterations (loss of heterozygosity (LOH) and microsatellite instability [MSI]) were examined using the crypt isolation method in glands from each tumor from patients with endometrial carcinoma. Twenty-five patients with endometrial adenocarcinoma who underwent surgery were included in this study. We obtained cancerous individual isolated tumor glands from each patient using the crypt isolation method. For LOH and MSI analyses, we used 15 microsatellite markers (3p, 5q, 10q, 13q, 17p, 18q, BAT25, and BAT26) and the promoter regions of 6 genes (transforming growth factor beta receptor II, BAX, insulin-like growth factor II receptor, E2F4, MutS homolog 3, and MSH6). Loss of heterozygosity was detected in 8 (32%) of 25 patients, and MSI was detected in 9 (36%) of 25 patients. Some MSI-positive carcinomas had LOH in single tumor gland samples, and the coexistence of LOH and MSI was confirmed. In 16 (64%) of 25 cases, intratumoral genetic heterogeneity amo...Continue Reading

References

Apr 16, 1998·International Journal of Cancer. Journal International Du Cancer·T H SuJ G Chang
Nov 26, 2002·Pathology International·Hiroshi TakahashiShin-ichi Nakamura
May 22, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Tamotsu SugaiMasanori Terashima
Jul 6, 2004·Journal of Gastroenterology·Motohiro IshiiShin-Ichi Nakamura
Sep 18, 2004·The Journal of Pathology·Yu-Fei JiaoShin-Ichi Nakamura
Jun 29, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·L H HonoréS E Andrew
Sep 18, 2008·International Journal of Cancer. Journal International Du Cancer·Yong Ho KimYoung S Kim
Feb 24, 2010·International Journal of Cancer. Journal International Du Cancer·Tamotsu SugaiKazuyuki Suzuki
May 3, 2013·Nature·Cyriac KandothDouglas A Levine

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