Molecular analysis of the bvg-repressed urease of Bordetella bronchiseptica

Microbial Pathogenesis
D J McMillanM J Walker

Abstract

Bordetella bronchiseptica is a ureolytic mammalian respiratory pathogen. We have investigated the regulation of urease in B. bronchiseptica and the potential role of this enzyme in eukaryotic invasion and intracellular survival. Our results indicate urease is a bordetella virulence repressed gene. Urease activity in virulent B. bronchiseptica BB7865 is up-regulated from basal levels by 5 gl1 magnesium sulphate at 37 degrees C. At 30 degrees C, urease activity remained at basal levels, even in the presence on magnesium sulphate, suggesting a second temperature dependent mechanism of urease regulation was also operating. Urease was not inducible by 10 mM urea nor up-regulated in nitrogen limiting conditions. To evaluate the role of urease in intracellular invasion and survival urease-negative mutants of B. bronchiseptica BB7865 and B. bronchiseptica BB7866 were created by transposon mutagenesis, and compared to the urease-positive parental strains in a HeLa cell invasion assay. We demonstrate that increasing the concentration of urea in the assay increased survival of the urease-positive but not urease-negative strains after 24 h, suggesting that urease does have a role in intracellular survival. Partial DNA sequence analysis of ...Continue Reading

Citations

Dec 21, 2000·Molecular Microbiology·K E StockbauerP A Cotter
Sep 18, 2001·Letters in Applied Microbiology·L E FriedmanM A Franco
Aug 12, 2009·Infection and Immunity·Tracy L NicholsonSusan L Brockmeier
Jun 18, 2002·Infection and Immunity·Ulrich HeiningerEric T Harvill
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Umesh AhujaJeff F Miller
Sep 17, 2015·Pathogens and Disease·Elizabeth A TrainorTod J Merkel
May 17, 2019·Journal of Breath Research·William R Bishai, Graham S Timmins
May 12, 2001·FEMS Microbiology Reviews·A M SmithM J Walker

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