Molecular analysis of the cyclin-dependent kinase inhibitor genes p15INK4b/MTS2, p16INK4/MTS1, p18 and p19 in human cancer cell lines

International Journal of Cancer. Journal International Du Cancer
A GemmaC C Harris

Abstract

Cyclin-dependent kinase-4 inhibitor genes (INK4) regulate the cell cycle and are candidate tumor-suppressor genes. To determine if alterations in the coding regions of the p18 and p19 genes, which are novel members of the INK4 family and if they correlate with the development of human cancer, 100 human cancer cell lines were analyzed. Two other INK4 gene family members, p15INK4b/MTS2 and p16INK4/MTS1 genes were also analyzed. Homozygous deletions of the p15INK4b/MTS2 gene were detected in 29 cancer cell lines. Thirty-five homozygous deletions and 7 intragenic mutations of the pl6INK4/MTS1 gene were also detected in these cell lines. Neither homozygous deletions nor intragenic mutations of the p18 and p19 genes were found except in an ovarian cancer cell line, SKOV3, harboring a single base pair deletion in exon 1 of p19. In p16INK4/MTS1 expression analysis, 5 cell lines with both authentic and alternative spliced p16INK4/MTS1 mRNA had no detectable p16INK4/MTS1 protein. These results suggest the hypotheses that either post-translational modification or enhanced degradation may be responsible for the lack of detection of the p16INK4/MTS1 protein. Using Western blot analysis, subsets of 26 human cancer cell lines were examined fo...Continue Reading

References

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Citations

Jul 21, 2004·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Akinobu YoshimuraShoji Kudoh
Sep 12, 2013·Cancer Biology & Therapy·Anna Felisiak-GolabekJolanta Kupryjanczyk
Sep 19, 2000·Genes, Chromosomes & Cancer·A GemmaS Kudoh
Oct 31, 2003·Molecular Carcinogenesis·Hu Xue JunShoji Kudoh
Jun 14, 2002·FEBS Letters·Youichirou MatsuzakiToshiyuki Sakai
Jul 2, 1999·British Journal of Cancer·J A Newton BishopD T Bishop
Jun 15, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Yvette P GeelsJohanna M A Pijnenborg
Oct 9, 2002·The Journal of Biological Chemistry·Ravichandran N VenkataramaniRonen Marmorstein
Apr 30, 2003·Virchows Archiv : an International Journal of Pathology·Britta KleistGerd Lorenz
Nov 21, 1998·Biochimica Et Biophysica Acta·M Ruas, G Peters

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