Molecular analysis of the DNA gyrB gene from Myxococcus xanthus.

Microbiology
Y PaitanElisha Orr

Abstract

DNA gyrase, an essential type II topoisomerase, mediates negative supercoiling of the bacterial chromosome, thereby affecting the processes of DNA replication, transcription, recombination and repair. The gyrB gene from the Gram-negative soil bacterium Myxococcus xanthus was sequenced. The sequence predicts a protein of 815 amino acid residues displaying significant homology to all known GyrB proteins. A 6-His-GyrB fusion protein was overexpressed in Escherichia coli and purified to near homogeneity using affinity chromatography on Ni-nitrilotriacetic acid-agarose and novobiocin-Sepharose columns. The fusion protein bound novobiocin and cross-reacted with anti-E. coli GyrB antibodies, indicating structural and functional similarities to the E. coli DNA GyrB. The gene was mapped to the region of the origin of replication (oriC) of M. xanthus.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1977·Journal of Bacteriology·G R JanssenM Dworkin
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M GellertJ Tomizawa
Oct 1, 1992·Antimicrobial Agents and Chemotherapy·M VaronE Rosenberg
Oct 1, 1992·Antimicrobial Agents and Chemotherapy·S TolchinskyE Rosenberg
Jan 1, 1991·Journal of Bacteriology·M L Holmes, M L Dyall-Smith
Mar 1, 1991·Journal of Bacteriology·H W ChenL J Shimkets
Jan 1, 1990·Methods in Enzymology·W R Pearson
Oct 11, 1990·Nucleic Acids Research·R E Parales, C S Harwood
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A KuspaD Kaiser
Oct 20, 1987·Journal of Molecular Biology·S L Swanberg, J C Wang
Jan 26, 1987·Nucleic Acids Research·T AdachiK Mizuuchi
Jan 1, 1984·Advances in Experimental Medicine and Biology·E OrrE Wahle
Aug 11, 1981·Nucleic Acids Research·W L Staudenbauer, E Orr
Jan 1, 1983·Molecular & General Genetics : MGG·L Avery, D Kaiser
Sep 1, 1994·Journal of Bacteriology·S A Ladefoged, G Christiansen
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Q HeL J Shimkets
May 1, 1994·Molecular Microbiology·E J Gilbert, A Maxwell
Jan 1, 1996·Annual Review of Biochemistry·J C Wang

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Citations

Apr 8, 2011·Journal of Agricultural and Food Chemistry·Yudai HottaHiroto Tamura
Feb 9, 2013·Canadian Journal of Microbiology·Niels Bohse Hendriksen, Jacob Carstensen
May 7, 2002·Canadian Journal of Microbiology·Niels Bohse Hendriksen, Bjarne Munk Hansen
Apr 4, 2003·Canadian Journal of Microbiology·Tatyana G YudinaGalina G Chestukhina
Mar 31, 2004·Canadian Journal of Microbiology·Marcus SchallmeyOwen P Ward
Aug 11, 2005·Canadian Journal of Microbiology·Lyudmila P RevinaAnna V Izumrudova
Aug 11, 2005·Canadian Journal of Microbiology·Rodolfo Quintana-CastroMario Ramírez-Lepe
Jun 1, 2007·Canadian Journal of Microbiology·Ameur CherifAbdellatif Boudabous
Aug 2, 2007·Canadian Journal of Microbiology·G T Vilas-BôasO M N Arantes
Jan 1, 2008·Biotechnology & Genetic Engineering Reviews·Maria Shanchez-Contreras, Isabella Vlisidou

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