PMID: 7538012Feb 1, 1995Paper

Molecular analysis of the endosymbionts of tsetse flies: 16S rDNA locus and over-expression of a chaperonin

Insect Molecular Biology
S Aksoy

Abstract

Based on 16S rDNA sequence comparison, intracellular mycetome-associated endosymbionts (P-endosymbionts) of tsetse flies (Diptera: Glossinidae) form a distinct lineage within the gamma-3 subdivision of proteobacteria, related to the free-living bacterium Escherichia coli, midgut S-endosymbionts of various insects including tsetse flies, and to the P-endosymbiont lineage of aphids, Buchnera aphidicola. Gene organization and expression of several loci in intracellular microorganisms have revealed differences from free-living bacteria. This study analyses two of these characteristics in tsetse endosymbionts; the copy number and gene organization of rDNA operations and the nature of the abundant protein(s) synthesized by these microorganisms. Results indicate that Glossina morsitans morsitans S-endosymbionts have multiple (seven) rDNA operons coding for 16S (rrs) followed by 23S (rrl) gene sequences, whereas tsetse P-endosymbionts have a single, similarly organized rDNA operon. In tsetse mycetocytes in vitro, P-endosymbionts synthesize a predominant protein of 60 kDa in size (p60) which by Western blot analysis shows immunological cross-reactivity with the abundant 63 kDa (p63) protein of B. aphidicola. p63 (also referred to as sym...Continue Reading

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Citations

Jul 25, 2003·Veterinary Parasitology·Serap Aksoy
Mar 19, 1999·Trends in Microbiology·J F van den HeuvelF van der Wilk
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L Akman, S Aksoy
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Feb 1, 2005·Insect Biochemistry and Molecular Biology·Youjia Hu, Serap Aksoy
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Jun 30, 2004·Trends in Microbiology·Rita V M RioSerap Aksoy
Aug 12, 2006·Cell·Colin Dale, Nancy A Moran
Jan 28, 2014·FEMS Microbiology Letters·Maria KupperRoy Gross
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