PMID: 8955298Dec 1, 1996Paper

Molecular analysis of the gat genes from Escherichia coli and of their roles in galactitol transport and metabolism

Journal of Bacteriology
B Nobelmann, J W Lengeler

Abstract

In enteric bacteria, the hexitol galactitol (Gat) (formerly dulcitol) is taken up through enzyme II (II(Gat)) of the phosphoenolpyruvate-dependent phosphotransferase system (PTS), and accumulated as galactitol 1-phosphate (Gat1P). The gat genes involved in galactitol metabolism have been isolated from the wild-type isolate Escherichia coli EC3132 and cloned on a 7.8-kbp PstI DNA fragment. They comprise six complete open reading frames and one truncated open reading frame in the order gatYZABCDR'. The genes gatABC code for the proteins GatA (150 residues) and GatB (94 residues), which correspond to the hydrophilic domains IIA(Gat) and IIB(Gat), and GatC, which represents a membrane-bound transporter domain IIC(Gat) (35 kDa, 427 residues). The three polypeptides together constitute a II(Gat) of average size (671 residues). Gene gatD codes for a Gat1P-specific NAD-dependent dehydrogenase (38 kDa, 346 residues), gatZ codes for a protein (42 kDa, 378 residues) of unknown function, and gatY (31 kDa, 286 residues) codes for a D-tagatose-1,6-bisphosphate aldolase with similarity to other known ketose-bisphosphate aldolases. The truncated gatR' gene, whose product shows similarity to the glucitol repressor GutR, closely resembles a gatR...Continue Reading

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Citations

Aug 10, 2000·Molecular Microbiology·A BrinkkötterJ W Lengeler
Feb 13, 2001·FEMS Microbiology Letters·S Otte, J W Lengeler
Sep 12, 2006·Protein Science : a Publication of the Protein Society·Laurent VolponKalle Gehring
Dec 24, 2010·Molecular Systems Biology·Shota AtsumiJames C Liao
Jul 29, 2011·Acta Crystallographica. Section D, Biological Crystallography·Karolina MichalskaAndrzej Joachimiak
Oct 19, 2014·Applied and Environmental Microbiology·James KirbyJay D Keasling
Jun 27, 2014·PLoS Genetics·Margie KinnersleyFrank Rosenzweig
Aug 31, 2018·Journal of Industrial Microbiology & Biotechnology·Qi Han, Mark A Eiteman
Nov 15, 2011·Journal of Bacteriology·Peter L FreddolinoSaeed Tavazoie
Feb 20, 2004·Nature·Ertugrul M OzbudakAlexander Van Oudenaarden
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shaobin ZhongAntony M Dean
May 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jerome T MettetalAlexander van Oudenaarden
Jan 26, 2005·Biochemical Society Transactions·M H SaierR D Barabote
Sep 20, 2011·Molecular Microbiology·Troy G HammerstromTheresa M Koehler
Mar 8, 2014·PLoS Genetics·João Barroso-BatistaIsabel Gordo
Mar 25, 2017·Frontiers in Cellular and Infection Microbiology·Muhammad AfzalSulman Shafeeq
Dec 12, 2018·Biotechnology and Bioengineering·Navneet RaiIlias Tagkopoulos
Jan 9, 2015·Applied Microbiology and Biotechnology·Kien Trung TranThomas K Wood
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Sep 6, 2006·Journal of Bacteriology·Tara K SigdelMichael W Crowder
Jun 15, 2007·Journal of Bacteriology·Fritz TitgemeyerMichael Niederweis
Feb 24, 2007·Applied Microbiology and Biotechnology·Michael SchümperliSven Panke
Nov 1, 2005·EcoSal Plus·Christoph Mayer, Winfried Boos

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