PMID: 9555906Apr 29, 1998Paper

Molecular analysis of the gene encoding F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis

Journal of Bacteriology
Endang Purwantini, Lacy Daniels

Abstract

The gene fgd, which codes for F420-dependent glucose-6-phosphate dehydrogenase (FGD), was cloned from Mycobacterium smegmatis, and its sequence was determined and analyzed. A homolog of FGD which has a very high similarity to the M. smegmatis FGD-derived amino acid sequence was identified in Mycobacterium tuberculosis. FGD showed significant homology with F420-dependent N5,N10-methylene-tetrahydromethanopterin reductase (MER) from methanogenic archaea and with several hypothetical proteins from M. tuberculosis and Archaeoglobus fulgidus, but FGD showed no significant homology with NADP-dependent glucose-6-phosphate dehydrogenases. Multiple alignment of FGD and MER proteins revealed four conserved consensus sequences. Multiple alignment of FGD with the hypothetical proteins also revealed portions of the same conserved sequences. Moderately high levels of FGD were expressed in Escherichia coli BL21(DE3) carrying fgd in pBluescript.

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Citations

Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Robert A Rohde, P Buford Price
Mar 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Endang Purwantini, Biswarup Mukhopadhyay
Nov 13, 2004·FEMS Microbiology Reviews·Iain C Sutcliffe, Dean J Harrington
Jan 16, 2010·The Journal of Biological Chemistry·Mohammad Rubayet HasanLacy Daniels
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Charles A Abbas, Andriy A Sibirny
May 18, 2016·Journal of Bacteriology·Endang PurwantiniBiswarup Mukhopadhyay
Apr 29, 2016·Microbiology and Molecular Biology Reviews : MMBR·Chris GreeningColin J Jackson
May 7, 2003·Applied and Environmental Microbiology·Gesche HeissHans-Joachim Knackmuss
Jul 13, 2005·Natural Product Reports·Markus Fischer, Adelbert Bacher
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert A RohdeNathan E Bramall
Oct 1, 2010·Environmental Microbiology Reports·Eric S BoydJohn W Peters
Dec 15, 2016·Applied Microbiology and Biotechnology·Quoc-Thai NguyenMarco W Fraaije
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·H C TungP B Price
Oct 20, 2005·Journal of Bioscience and Bioengineering·Seigo ShimaUlrich Ermler
Apr 15, 2021·FEMS Microbiology Reviews·Rhys Grinter, Chris Greening
Jun 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Wassihun Wedajo AragawThomas Dick

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