Molecular analysis of the macrolide-lincosamide resistance gene region of a novel plasmid from Staphylococcus hyicus

Journal of Medical Microbiology
Stefan SchwarzC Werckenthin

Abstract

Resistance to macrolides and lincosamides in Staphylococcus hyicus has been shown to be encoded by a 4.0-kb plasmid designated pSES21. It differed distinctly in its restriction map from all other staphylococcal macrolide resistance plasmids reported so far. Southern blot hybridisation with gene probes specific for staphylococcal erm genes demonstrated that the macrolide resistance gene belonged to hybridisation class C. Analysis of the ermC gene revealed that the deduced amino-acid sequence of the pSES21-encoded ErmC methylase exhibited c. 93% identity with the ErmC methylase encoded by plasmid pE194. The ermC gene of pSES21 was expressed constitutively and sequence analysis of the regulatory region showed multiple base-pair insertions and substitutions in the translational attenuator. As a consequence of these mutations, the reading frame of the small regulatory peptide was destroyed and a novel pair of inverted repeated sequences was generated. Previous studies identified sequence deletions and sequence duplications in the ermC regulatory region as the basis for constitutive ermC gene expression. The multiple point mutations shown in the pSES21-encoded ermC translational attenuator represent a novel kind of structural alterat...Continue Reading

Citations

Oct 5, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Franz-Josef SchmitzStefan Schwarz
Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Dec 24, 2011·Annals of the New York Academy of Sciences·Stefan SchwarzKristina Kadlec
Mar 19, 2013·International Journal of Medical Microbiology : IJMM·Sarah WendlandtKristina Kadlec
Jul 21, 2004·Veterinary Microbiology·Dieter VancraeynestFreddy Haesebrouck
Apr 20, 2010·Veterinary Microbiology·Ramona Stegmann, Vincent Perreten
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·C WerckenthinH Westh
Jul 12, 2018·Microbiology Spectrum·Stefan SchwarzJianzhong Shen

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