Molecular analysis of the microflora associated with dental caries

Journal of Clinical Microbiology
M A MunsonW G Wade

Abstract

Molecular techniques have revealed many novel, presumed unculturable, taxa in oral infections. The aim of this study was to characterize the bacterial community of the middle and advancing front of carious dental lesions by cultural and molecular analyses. Samples were collected with a hand excavator from five teeth with carious lesions involving dentine. Samples were cultured on blood agar and Rogosa agar incubated in air plus 5% CO(2) and on fastidious anaerobe agar anaerobically. DNA was also extracted directly from the samples and 16S rRNA genes were amplified by PCR with universal primers. PCR products were singularized by cloning, and the cloned inserts and cultured isolates were identified by 16S rRNA gene sequence analysis. We identified 95 taxa among the 496 isolates and 1,577 clones sequenced; 44 taxa were detected by the molecular method alone; 31 taxa were previously undescribed. Only three taxa, Streptococcus mutans, Rothia dentocariosa, and an unnamed Propionibacterium sp., were found in all five samples. The predominant taxa by anaerobic cultivation were the novel Propionibacterium sp. (18%), Olsenella profusa (14%), and Lactobacillus rhamnosus (8%). The predominant taxa in the molecular analysis were Streptococc...Continue Reading

References

Oct 1, 1985·Journal of Dental Research·E Hoshino
Jan 1, 1973·Caries Research·W J Loesche, S A Syed
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Mar 1, 1996·Journal of Clinical Microbiology·D DymockW G Wade
Oct 6, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W G WadeA J Weightman
Oct 6, 1998·Applied and Environmental Microbiology·M F Polz, C M Cavanaugh
Jan 20, 1999·Caries Research·K L WeerheijmW E van Amerongen
May 24, 2001·Journal of Bacteriology·B J PasterF E Dewhirst
Mar 7, 2002·Journal of Clinical Microbiology·Mitzi R BeckerAnn L Griffen
Oct 31, 2002·Journal of Dental Research·M A MunsonW G Wade

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Citations

Sep 16, 2010·European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry·S KneistB Thiede
Nov 29, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D PrezaB J Paster
Dec 22, 2004·Current Infectious Disease Reports·Mark W Hull, Anthony W Chow
Mar 31, 2009·Folia Microbiologica·P SvecM Kukletová
Nov 29, 2007·The ISME Journal·Stuart P DonachieMark V Brown
Jun 15, 2012·Photomedicine and Laser Surgery·Natália Costa AraújoMarleny Elizabeth Martinez Gerbi
Dec 7, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mouhamad Al MasalmaPierre-Edouard Fournier
Sep 8, 2011·Journal of Biomedical Optics·Camila de Almeida B GuglielmiMaria A A C Luz
Jun 10, 2008·Applied and Environmental Microbiology·M Andrea Azcarate-PerilTodd R Klaenhammer
Aug 30, 2008·Applied and Environmental Microbiology·David BeightonJennifer Gallagher
Apr 7, 2009·Applied and Environmental Microbiology·S R VartoukianW G Wade
Sep 29, 2011·Applied and Environmental Microbiology·Zhoujie XieJustin Merritt
Jul 27, 2010·Journal of Bacteriology·Floyd E DewhirstWilliam G Wade
Aug 8, 2006·Journal of Clinical Microbiology·Hans-Peter HorzGeorg Conrads
Sep 10, 2010·Journal of Clinical Microbiology·Erin L GrossAnn L Griffen
Jan 25, 2008·Journal of Clinical Microbiology·Jørn A AasBruce J Paster
Nov 3, 2006·Journal of Clinical Microbiology·Y LiP W Caufield
Mar 5, 2010·Journal of Clinical Microbiology·Mangala A NadkarniNeil Hunter
Apr 4, 2008·Journal of Clinical Microbiology·Dorita PrezaBruce J Paster
Feb 4, 2011·Journal of Clinical Microbiology·A C R TannerF E Dewhirst
Nov 8, 2005·Journal of Clinical Microbiology·P M CorbyB J Paster
Feb 8, 2005·Journal of Clinical Microbiology·Kim-Ly ChhourNeil Hunter
Jul 8, 2005·Journal of Clinical Microbiology·J F Siqueira, I N Rôças
Aug 6, 2005·Journal of Clinical Microbiology·Purnima S KumarEugene J Leys
May 5, 2006·Journal of Clinical Microbiology·Samuel J HooperMelanie J Wilson
Jan 11, 2011·Canadian Journal of Microbiology·Guillaume G Nicolas, Marc C Lavoie
Feb 18, 2011·Canadian Journal of Microbiology·Christopher D Sibley, Michael G Surette
Sep 24, 2010·Caries Research·E KanasiA C R Tanner
Feb 19, 2010·Journal of Dental Research·E KanasiA C R Tanner
Feb 26, 2008·Journal of Dental Research·N ArifD Beighton
Jan 11, 2007·Bioscience, Biotechnology, and Biochemistry·Kenichi HojoNobuko Maeda
Oct 4, 2011·PloS One·Sebastian SchlaferBente Nyvad
Mar 23, 2013·PloS One·Scott N PetersonWalter Bretz
Jan 9, 2008·Twin Research and Human Genetics : the Official Journal of the International Society for Twin Studies·Patricia M CorbyBruce J Paster
Mar 5, 2008·Journal of Periodontology·G Rutger PerssonRigmor E Persson
Jan 27, 2012·Journal of Oral Microbiology·José F SiqueiraIsabela N Rôças

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