Molecular analysis of the Pseudomonas aeruginosa genes, ruvA, ruvB and ruvC, involved in processing of homologous recombination intermediates

Gene
T HishidaH Shinagawa

Abstract

In Escherichia coli, the products of the ruvA, ruvB and ruvC genes are all involved in the processing of recombination intermediates (Holliday structures) into recombinant molecules. We cloned a 9.4-kb DNA fragment from Pscudomonas aeruginosa PAO1 in a plasmid by functional complementation of the UV sensitivity of an E. coli strain with ruvABC deleted. In P. aeruginosa, the ruv region seemed to form a non-SOS regulated single operon consisting of orf26-ruvC-ruvA-ruvB, while in this region of E. coli, ruvA and ruvB form an SOS-regulated operon, orf26 and ruvC form a non-SOS operon, and these two operons are split by orf23. The deduced amino acid sequences of P. aeruginosa RuvA, RuvB and RuvC proteins were 55, 72 and 55% identical to those of the corresponding E. coli Ruv proteins. The individual ruv genes of P. aeruginosa complemented the corresponding single ruv mutations of E. coli, suggesting that the P. aeruginosa Ruv proteins can interact functionally with their E. coli Ruv partners in forming heterologous complexes. The sequence alignments of the Ruv proteins were extended by incorporation of data about the putative ruv genes obtained from data banks, and the RuvB sequences were conspicuously more conserved than the RuvA a...Continue Reading

References

Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B ConnollyS C West
Jan 1, 1990·Annual Review of Microbiology·R V Miller, T A Kokjohn
Apr 27, 1989·Nature·W R Brown
May 1, 1989·Journal of Bacteriology·C B RussellF W Dahlquist
Oct 1, 1989·Molecular & General Genetics : MGG·H IwasakiH Shinagawa
Jul 1, 1987·Molecular & General Genetics : MGG·Y Sano, M Kageyama
Aug 1, 1985·Journal of Bacteriology·T A Kokjohn, R V Miller
Mar 1, 1986·Journal of Bacteriology·I Goldberg, J J Mekalanos
Apr 1, 1986·Molecular & General Genetics : MGG·K PaulJ Das
Jan 11, 1988·Nucleic Acids Research·R E Rose
Dec 1, 1972·Bacteriological Reviews·B J Bachmann
Sep 1, 1969·Bacteriological Reviews·B W Holloway
Dec 21, 1984·Nucleic Acids Research·A Bernardi, F Bernardi
Jul 1, 1983·Journal of Bacteriology·M Better, D R Helinski
Jan 1, 1982·Molecular & General Genetics : MGG·C E Shurvinton, R G Lloyd
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Oct 20, 1995·Science·C M FraserJ C Venter
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·A SaitoH Shinagawa
Sep 1, 1994·Microbiological Reviews·S C KowalczykowskiW M Rehrauer
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·I R TsanevaS C West
Mar 1, 1996·Trends in Biochemical Sciences·H Shinagawa, H Iwasaki

❮ Previous
Next ❯

Citations

Oct 10, 1998·Research in Microbiology·C Boursaux-EudeI Saints Girons
Jan 1, 1997·Annual Review of Genetics·S C West
Mar 4, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tatsuya ShibataHideo Shinagawa
Oct 17, 1998·Molecular Cell·S M RoeL H Pearl
Nov 2, 1999·Journal of Molecular Biology·H GeorgeS C West
Nov 24, 2004·The Journal of Biological Chemistry·Cyril V PrivezentzevIrina R Tsaneva
Jun 24, 2005·The Journal of Biological Chemistry·Takayuki OhnishiHideo Shinagawa
Feb 3, 1998·Mutation Research·S KitayamaH Itoh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.