Molecular analysis of the replication elements of the broad-host-range RepA/C replicon

Plasmid
C LlanesP Plesiat

Abstract

RepA/C is a replicon specific to the IncA/C incompatibility group of plasmids and was isolated recently from plasmid RA1. The sequence of this autoreplicative region was established; it contains 13 repeats, suggesting that the replicon uses iterons to control its copy number. The sequence contains two ORFs, one potentially coding for a 33-kDa protein (ORF1) and a second potentially coding for a 14-kDa protein (ORF2) (Llanes et al., 1994b). In this work, using an in vitro transcription/translation system, we detected a polypeptide whose size corresponded well to that of the deduced product of ORF1. Deletion and insertion mutation analysis showed that ORF1 is essential for replication; it encodes an initiator protein (called RepA). ORF2 was not essential for replication in Escherichia coli and its function remains to be determined. Using complementation experiments, the replication origin (ori) of RepA/C was defined. The ori was located in a 600-bp fragment downstream from repA, containing 10 direct repeats. To study the control of repA expression, a transcriptional fusion PrepA::lacZ was constructed. Its analysis showed that repA is transcriptionally autoregulated as are most repA genes of replicons controlled by iterons.

Citations

Jun 12, 2013·Applied and Environmental Microbiology·R J MeinersmannB B Oakley
Jun 2, 2009·Journal of Bacteriology·W Florian FrickeJacques Ravel
Sep 21, 2010·Journal of Bacteriology·Haruo SuzukiEva M Top
Dec 31, 2009·PLoS Genetics·Rachel A F WozniakMatthew K Waldor
Sep 9, 2015·Frontiers in Microbiology·Dominic Poulin-LapradeVincent Burrus
Apr 26, 2015·Plasmid·Christopher J Harmer, Ruth M Hall
Jul 4, 2012·Mobile Genetic Elements·Timothy J Johnson, Kevin S Lang
Apr 13, 2007·The Journal of General and Applied Microbiology·Daisuke InoueMasanori Fujita
Aug 31, 2019·Annals of the New York Academy of Sciences·Katlego KopotsaNontombi Marylucy Mbelle
Mar 1, 2012·Journal of Aquatic Animal Health·Benjamin R LafrentzPhillip H Klesius
Feb 3, 2011·Journal of Environmental Quality·Tatsuya AkiyamaMary C Savin
Jan 5, 1999·Applied and Environmental Microbiology· Van Ham RCHJA Latorre
Feb 14, 2003·Plasmid·Miranda SherleyPeter J Collignon
Jun 7, 2005·Journal of Microbiological Methods·Alessandra CarattoliE John Threlfall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.