PMID: 11900843Mar 20, 2002Paper

Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates

Virus Research
Mustafa HasoksuzLinda Saif

Abstract

It is unclear whether respiratory and enteric bovine coronavirus (BoCV) strains are distinctive in biological, antigenic and genetic characteristics. In the present study, we analyzed the nucleotide and amino acid sequence of the S1 subunit of the S glycoprotein, including the cleavage site, of both respiratory (n=5) and enteric (n=3) BoCV isolates including two paired isolates from the same feedlot animals and compared them with the prototype Mebus and two enteric and one respiratory BoCV strains from Quebec. A total of 75 polymorphic nucleotides were identified in the S1 subunit of the spike glycoprotein of BoCV isolates compared with the Mebus strain. These polymorphisms led to 42 amino acid changes at 38 distinct sites. The amino acid changes were distributed throughout the S1 subunit with clustering around residues 40-118, 146-179, and 458-531. Among these variations, only 19 amino acid substitutions altered the charge, hydrophobicity and surface probability of the protein. Based on phylogenetic analysis, our respiratory and enteric isolates clustered into two major groups with two subgroups. Although, there were only a few amino acid changes between the respiratory and enteric paired isolates, the other two respiratory is...Continue Reading

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Aug 3, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mustafa HasoksuzLinda J Saif

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Citations

Mar 7, 2006·Journal of Clinical Microbiology·Lihong LiuSándor Belák
Aug 3, 2006·American Journal of Veterinary Research·Christopher J ThomasLinda J Saif
Mar 13, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nathalie KinMeriadeg Ar Gouilh
Jun 7, 2007·The Journal of Veterinary Medical Science·Rikio KirisawaHiroshi Iwai
Feb 6, 2009·The Journal of Veterinary Medical Science·Toru KannoIkuo Uchida
Jul 4, 2012·The Veterinary Journal·Mehdi R M BidokhtiStefan Alenius
May 29, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nadia MartínezLester J Pérez
Apr 9, 2008·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E TakiuchiA A Alfieri
Sep 19, 2009·Journal of Dairy Science·S M GulliksenO Østerås
Apr 30, 2020·Veterinary Medicine and Science·Samad LotfollahzadehDavid George Watson
Feb 17, 2019·Virus Genes·Jihye ShinDong-Jun An
Jul 20, 2017·Tropical Animal Health and Production·Kanokwan SingasaPipat Arunvipas
Jul 19, 2020·Tropical Animal Health and Production·Jaka Jakob HodnikJože Starič
Dec 16, 2020·Gene Therapy·Burak BerberCihan Tastan
Apr 20, 2021·Frontiers in Veterinary Science·Anastasia N Vlasova, Linda J Saif

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