Molecular analysis of three Ljungan virus isolates reveals a new, close-to-root lineage of the Picornaviridae with a cluster of two unrelated 2A proteins

Journal of Virology
Susanne JohanssonA M Lindberg

Abstract

Ljungan virus (LV) is a suspected human pathogen recently isolated from bank voles (Clethrionomys glareolus). In the present study, it is revealed through comparative sequence analysis that three newly determined Swedish LV genomes are closely related and possess a deviant picornavirus-like organization: 5' untranslated region-VP0-VP3-VP1-2A1-2A2-2B-2C-3A-3B-3C-3D-3' untranslated region. The LV genomes and the polyproteins encoded by them exhibit several exceptional features, such as the absence of a predicted maturation cleavage of VP0, a conserved sequence determinant in VP0 that is typically found in VP1 of other picornaviruses, and a cluster of two unrelated 2A proteins. The 2A1 protein is related to the 2A protein of cardio-, erbo-, tescho-, and aphthoviruses, and the 2A2 protein is related to the 2A protein of parechoviruses, kobuviruses, and avian encephalomyelitis virus. The unprecedented association of two structurally different 2A proteins is a feature never previously observed among picornaviruses and implies that their functions are not mutually exclusive. Secondary polyprotein processing of the LV polyprotein is mediated by proteinase 3C (3C(pro)) possessing canonical affinity to Glu and Gln at the P1 position and ...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T HyypiäG Stanway
Sep 1, 1990·The Journal of General Virology·P Auvinen, T Hyypiä
Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Jan 1, 1990·Annual Review of Microbiology·A C Palmenberg
Jan 1, 1989·Annual Review of Biochemistry·M G Rossmann, J E Johnson
Jan 1, 1988·Annual Review of Biochemistry·H G Kräusslich, E Wimmer
Jan 1, 1974·Intervirology·J L MelnickH A Wenner
Nov 4, 1994·Journal of Molecular Biology·S Henikoff, J G Henikoff
Jan 1, 1993·Annual Review of Genetics·E WimmerX Cao
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·F LiB S Crabb
Aug 1, 1996·The Journal of General Virology·T PöyryG Stanway
Oct 1, 1996·Journal of Virology·H Hahn, A C Palmenberg
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Nov 1, 1996·Protein Science : a Publication of the Protein Society·N BlomS Brunak
Jan 1, 1997·The Journal of General Virology·M L DonnellyM D Ryan
Jan 1, 1997·Methods in Molecular Biology·D Bertioli
Apr 1, 1997·The Journal of General Virology·M D Ryan, M Flint
May 1, 1997·Journal of Virological Methods·A M LindbergS Johansson
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·K Strimmer, A von Haeseler
Aug 22, 1997·The Journal of Biological Chemistry·T PulliT Hyypiä
Nov 20, 1998·The Journal of General Virology·F GhaziG Stanway
Mar 26, 1999·The Journal of General Virology·P MarvilD Cavanagh
Jan 29, 2000·Nucleic Acids Research·J H ChenJ V Maizel
Mar 22, 2000·The Journal of General Virology·J ZiebuhrA E Gorbalenya
Jun 14, 2000·Journal of Virology·K TriantafilouN Fernandez
Jun 22, 2000·The Journal of General Virology·P Joki-KorpelaT Hyypiä
Jul 4, 2001·Journal of Virology·H Hahn, A C Palmenberg
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

Citations

Nov 10, 2010·Nature Reviews. Microbiology·Vadim I Agol, Anatoly P Gmyl
Mar 15, 2013·Molecular Biology and Evolution·Etienne Simon-Loriere, Edward C Holmes
Jun 6, 2008·Journal of Clinical Microbiology·W Allan NixM Steven Oberste
Jul 13, 2012·Journal of Virology·Virginie SauvageMarc Eloit
Oct 19, 2007·Journal of Virology·A KapoorEric L Delwart
Jun 18, 2010·Journal of Virology·Jani SeitsonenSarah J Butcher
Jul 17, 2009·BMC Evolutionary Biology·Nuno R FariaLia van der Hoek
Aug 27, 2013·PloS One·Saskia L SmitsBart L Haagmans
May 15, 2013·Archives of Virology·Anne-Marie SalisburyJames P Stewart
May 15, 2012·Journal of Virological Methods·René KalliesMatthias Niedrig
Jan 18, 2011·Antiviral Research·Heléne NorderRolf Hilgenfeld
Oct 20, 2009·Molecular Phylogenetics and Evolution·Nicole Lewis-Rogers, Keith A Crandall
Jul 16, 2013·Journal of Medical Virology·Anne J JääskeläinenOlli Vapalahti
Apr 12, 2008·Journal of Virological Methods·Conny TolfA M Lindberg
Feb 13, 2007·Virus Research·Chun-Hsien Tseng, Hsiang-Jung Tsai
Sep 17, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kieran C PounderLorraine M McElhinney
Oct 5, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Henry F Krous, Neil E Langlois
Mar 20, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Lili ZhengHong Xin
Mar 7, 2009·The Journal of General Virology·Ciğdem H WilliamsG Stanway
Dec 18, 2013·The Journal of General Virology·Marisa BarbknechtMichael A Hoffman
Oct 4, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ákos BorosGábor Reuter
Feb 16, 2007·Virology·Chunyu Ding, Dabing Zhang
Jun 17, 2014·Veterinary Microbiology·Qinfeng LiaoDabing Zhang
May 22, 2016·The Journal of General Virology·Hiromichi MitakeMakoto Sugiyama
Apr 20, 2004·Biochemical and Biophysical Research Communications·E Susanne JohanssonA M Lindberg
Aug 9, 2006·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·A SamsioeB Niklasson
Mar 6, 2007·Birth Defects Research. Part A, Clinical and Molecular Teratology·B NiklassonW Klitz
Oct 9, 2015·Nature Communications·Ling ZhuDavid I Stuart
Feb 22, 2007·Journal of Medical Virology·Sisko TauriainenHeikki Hyöty
Aug 9, 2017·Frontiers in Microbiology·Xiaoyao YangXiaoyue Chen
Sep 30, 2020·Archives of Microbiology·T G VillaA Sánchez-Pérez
Jun 4, 2020·Vector Borne and Zoonotic Diseases·Cristina FevolaHeidi C Hauffe

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.