Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells.

Virus Research
Junji XingChunfu Zheng

Abstract

The herpes simplex virus type I (HSV-1) US11 protein is an RNA-binding multifunctional regulator that specifically and stably associates with nucleoli. Although the C-terminal part of US11 was responsible for its nucleolar localization, the precise nucleolar localization signal (NoLS) and nuclear export signal (NES) of US11 and its nuclear import and export mechanisms are still elusive. In this study, fluorescence microscopy was employed to investigate the subcellular localization of US11 and characterize its transport mechanism in living cells. By constructing a series of deletion mutants fused with enhanced yellow fluorescent protein (EYFP), three novel NoLSs of US11 were for the first time mapped to amino acids 84-125, 126-152, and 89-146, respectively. Additionally, the NES was identified to locate between amino acids 89 and 119. Furthermore, the US11 protein was demonstrated to target to the cytoplasm through the NES by chromosomal region maintenance 1 (CRM1)-independent pathway, and to the nucleolus through Ran and importin beta-dependent mechanism that does not require importin alpha 5.

References

May 1, 1996·The Journal of Cell Biology·I PalaciosC Dingwall
Mar 7, 1997·The Journal of Biological Chemistry·N C ChiS A Adam
Oct 10, 1997·Cell·K S UllmanD J Forbes
Dec 28, 1999·Annual Review of Cell and Developmental Biology·A Kaffman, E K O'Shea
Jan 26, 2002·Molecular and Cellular Biology·Frédéric CatezJean-Jacques Diaz
Mar 22, 2002·The Journal of General Virology·Marieke A TijmsEric J Snijder
Jul 12, 2002·Archives of Virology·J A Hiscox
Aug 16, 2002·Molecular Biology of the Cell·Katinka DöhnerBeate Sodeik
Mar 27, 2003·Traffic·Janna BednenkoLarry Gerace
Apr 20, 2005·Journal of Cellular Biochemistry·Wei HuDavid A Jans
Sep 6, 2005·Journal of Virology·Chunfu ZhengSylvia van Drunen Littel-van den Hurk
Sep 29, 2006·Proceedings of the National Academy of Sciences of the United States of America·James R Boyne, Adrian Whitehouse
Apr 24, 2007·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·D Hoeller Obrigkeit, J M Baron
May 22, 2007·Traffic·Daniela Martino RothDavid A Jans
May 24, 2007·Nature Reviews. Molecular Cell Biology·François-Michel BoisvertAngus I Lamond
Jul 8, 2008·Journal of Biomedical Optics·Richard N Day, Fred Schaufele
Feb 21, 2009·EMBO Reports·Edward Emmott, Julian A Hiscox

❮ Previous
Next ❯

Citations

Oct 16, 2010·Medical Microbiology and Immunology·Junji XingChunfu Zheng
Oct 6, 2012·Virus Research·Timothy L LochmannLeslie J Parent
Jun 12, 2019·Cells·Tanner M TessierJoe S Mymryk
Aug 10, 2021·Frontiers in Microbiology·Jingjing LiMeili Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
GQ999614

Methods Mentioned

BETA
two-hybrid
environmental stress
PCR
transfection
fluorescence microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.