Molecular and biochemical characterization of cytosolic phosphoglucomutase in maize. Expression during development and in response to oxygen deprivation

Plant Physiology
S ManjunathJ Bailey-Serres

Abstract

Phosphoglucomutase (PGM) catalyzes the interconversion of glucose (Glc)-1- and Glc-6-phosphate in the synthesis and consumption of sucrose. We isolated two maize (Zea mays L.) cDNAs that encode PGM with 98.5% identity in their deduced amino acid sequence. Southern-blot analysis with genomic DNA from lines with different Pgm1 and Pgm2 genotypes suggested that the cDNAs encode the two known cytosolic PGM isozymes, PGM1 and PGM2. The cytosolic PGMs of maize are distinct from a plastidic PGM of spinach (Spinacia oleracea). The deduced amino acid sequences of the cytosolic PGMs contain the conserved phosphate-transfer catalytic center and the metal-ion-binding site of known prokaryotic and eukaryotic PGMs. PGM mRNA was detectable by RNA-blot analysis in all tissues and organs examined except silk. A reduction in PGM mRNA accumulation was detected in roots deprived of O2 for 24 h, along with reduced synthesis of a PGM identified as a 67-kD phosphoprotein on two-dimensional gels. Therefore, PGM is not one of the so-called "anaerobic polypeptides." Nevertheless, the specific activity of PGM was not significantly affected in roots deprived of O2 for 24 h. We propose that PGM is a stable protein and that existing levels are sufficient to...Continue Reading

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Citations

Mar 23, 2006·Functional & Integrative Genomics·Huazong ZengLijun Luo
May 2, 2012·Journal of Proteome Research·Pedro E Lázaro-MixtecoTzvetanka D Dinkova
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·S ManjunathJ Bailey-Serres
Mar 9, 2013·Bioscience, Biotechnology, and Biochemistry·Brian P O'NeillStevens M Brumbley
Jul 9, 2004·Protein Science : a Publication of the Protein Society·Grant S ShackelfordLesa J Beamer
Feb 21, 2007·Plant Biotechnology Journal·Barrie Fong ChongStevens M Brumbley
Nov 6, 2009·Molecular Systems Biology·Maria PiquesMark Stitt
Jan 1, 2010·Annual Review of Food Science and Technology·Peter L Keeling, Alan M Myers
Mar 16, 2012·Plant Physiology and Biochemistry : PPB·Chiara PucciarielloPierdomenico Perata
Jul 28, 2017·The New Phytologist·Erin L McAdamJohn J Ross
Jul 13, 2012·Bioscience, Biotechnology, and Biochemistry·Kimio UematsuHideaki Yukawa
Jun 10, 2017·Frontiers in Plant Science·Clarice A MegguerFernando L Finger
Sep 29, 2020·The New Phytologist·Tobias MeitzelLjudmilla Borisjuk
Feb 7, 2006·Journal of Proteome Research·Ghulam Muhammad Ali, Setsuko Komatsu

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