Molecular and biochemical expression of TLRs in human amniotic membrane: a comparative study of fresh and cryopreserved specimens

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Alessandra MiceraStefano Bonini

Abstract

To assess the expression of toll-like receptors (TLRs) in human amniotic membrane (AM) specimens and compare this expression with those of AMs undergoing the standard preservation procedure (handling) for ocular surgery. Human fresh (n = 10; five spontaneous and five cesarean) or handled (n = 5) AMs were analyzed for TLR gene and protein expression. Two pieces were obtained from each specimen, and subjected to molecular or biochemical analysis. Relative real-time PCR and SDS-PAGE were carried out according to standard procedures. The REST-ANOVA coupled analysis was used to compare the molecular and biochemical data. The fresh membranes expressed all the TLRs (TLR1-10), with different gene expression as detected/evidenced by the Ct values, the intra-fresh group analysis showing that there was a variation of TLR expression whichvaried within the fresh membranes. The handled AMs retained the TLR expression after standard processing and preservation, but with a particular pattern which included a high TLR3/TLR4 and low TLR6 expression, when compared to the fresh membranes. The molecular data were confirmed by Western blot analysis. AM is routinely used in several ophthalmic surgical procedures, and notwithstanding its preservation ...Continue Reading

References

Mar 1, 1997·American Journal of Ophthalmology·S H Lee, S C Tseng
Dec 24, 1997·American Journal of Ophthalmology·S C TsengS H Lee
Aug 6, 1999·The British Journal of Ophthalmology·A Azuara-BlancoH S Dua
Dec 3, 1999·Klinika oczna·J Markuszewska, P Krzyzanowska
Dec 30, 1999·Progress in Retinal and Eye Research·J ImanishiS Kinoshita
Jul 25, 2000·The British Journal of Ophthalmology·H J ChenS C Tseng
Nov 4, 2000·Transplantation·W J KingA J George
Feb 13, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·N KjaergaardH Madsen
Aug 17, 2001·Current Opinion in Ophthalmology·K C SippelC S Foster
Mar 5, 2003·Advances in Experimental Medicine and Biology·Shigeru KinoshitaKohji Nishida
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiroyuki NagaseKoichi Hirai
Jan 9, 2004·Survey of Ophthalmology·Harminder S DuaV Senthil Maharajan
Sep 15, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Marco CoassinStefano Bonini
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Andrew HopkinsonHarminder S Dua
Jan 2, 2007·Cornea·Jean-Jacques GicquelPaul Dighiero
Jan 12, 2007·The Ocular Surface·Scheffer C G TsengChia-Yang Liu
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takayuki ImanishiTakashi Saito
Oct 2, 2007·BJOG : an International Journal of Obstetrics and Gynaecology·S PatniC A Thornton
Sep 30, 2008·Tissue Engineering. Part C, Methods·Andrew HopkinsonHarminder S Dua
Jan 29, 2011·Clinical Science·Alessandro LambiaseStefano Bonini
Mar 26, 2011·Expert Review of Ophthalmology·Jingbo LiuScheffer Cg Tseng
Jul 22, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claire GillauxMichelle Breuiller-Fouché
May 18, 2012·Cell and Tissue Research·A C MamedeM F Botelho
Aug 29, 2012·Cell and Tissue Banking·Emanuele RicciOrnella Parolini
Sep 13, 2012·American Journal of Reproductive Immunology : AJRI·Vikki M AbrahamsRamkumar Menon

❮ Previous
Next ❯

Citations

Sep 13, 2015·Cell and Tissue Banking·Natasha M J PerepelkinJanet A W Elliott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.