Molecular and cellular profiling of acute responses to total body radiation exposure in ovariectomized female cynomolgus macaques

International Journal of Radiation Biology
Ryne J DeBoJ Mark Cline

Abstract

The threat of radiation exposure requires a mechanistic understanding of radiation-induced immune injury and recovery. The study objective was to evaluate responses to ionizing radiation in ovariectomized (surgically post-menopausal) female cynomolgus macaques. Animals received a single total-body irradiation (TBI) exposure at doses of 0, 2 or 5 Gy with scheduled necropsies at 5 days, 8 weeks and 24 weeks post-exposure. Blood and lymphoid tissues were evaluated for morphologic, cellular, and molecular responses. Irradiated animals developed symptoms of acute hematopoietic syndrome, and reductions in thymus weight, thymopoiesis, and bone marrow cellularity. Acute, transient increases in plasma monocyte chemoattractant protein 1 (MCP-1) were observed in 5 Gy animals along with dose-dependent alterations in messenger ribonucleic acid (mRNA) signatures in thymus, spleen, and lymph node. Expression of T cell markers was lower in thymus and spleen, while expression of macrophage marker CD68 (cluster of differentiation 68) was relatively elevated in lymphoid tissues from irradiated animals. Ovariectomized female macaques exposed to moderate doses of radiation experienced increased morbidity, including acute, dose-dependent alterations...Continue Reading

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Citations

Mar 24, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Abdur Rahman Ansari, Huazhen Liu
Mar 2, 2019·International Journal of Radiation Biology·Angela M Groves, Jacqueline P Williams
Jul 21, 2021·Life Sciences in Space Research·Rajeev I DesaiCharles L Limoli

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