Molecular and cellular studies of tryptophanyl-tRNA synthetase using monoclonal antibodies. Evaluation of a common antigenic determinant in eukaryotic, prokaryotic and archaebacterial enzymes which maps outside the catalytic domain.

European Journal of Biochemistry
S F BerestenL L Kisselev

Abstract

Monoclonal antibodies referred to as Am1, Am2 and Am3 against highly purified bovine tryptophanyl-tRNA synthetase were prepared. Am2 antibodies inhibit the Trp-tRNA synthetase activity and interact with the active truncated enzyme forms (dimers of either 40-kDa or 51-kDa fragments) produced by limited proteolysis. Am1 and Am3 antibodies exert no effect on the Trp-tRNA synthetase activity; epitopes recognized by them are mapped close to one another and reside at the dispensable part of the Trp-tRNA synthetase molecule. Am1 cross-reacts with Trp-tRNA synthetases of eukaryotic, prokaryotic and archaebacterial species, as revealed by immunoblot analysis. A rapid two-step technique was developed for isolating electrophoretically homogeneous Trp-tRNA synthetase from Escherichia coli. The purified enzyme interacted with Am1, but not with Am2 and Am3 antibodies taken at the same concentrations. As in the case of eukaryotic Trp-tRNA synthetase, Am1 did not influence the activity of Trp-tRNA synthetase from E. coli. From the aforementioned results it follows that: (a) the conservation of part of the Trp-tRNA synthetase structure which is not directly involved in the formation of the catalytic centre of prokaryotic and eukaryotic Trp-tRNA...Continue Reading

References

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Citations

Jan 1, 1995·International Journal of Immunopharmacology·J BrochierB Klein
Jan 1, 1993·Biochimie·L L Kisselev
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M J RogersD Söll
Apr 11, 1992·Nucleic Acids Research·S BerestenD Söll
Dec 24, 2014·International Journal of Molecular Sciences·Adam C MirandoKaren M Lounsbury
May 29, 2000·The Journal of Biological Chemistry·R JorgensenJ Justesen
Dec 1, 1995·Immunology Letters·E L PaleyL Smelansky

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