Molecular and culture-based assessment of the microbial diversity of diabetic chronic foot wounds and contralateral skin sites.

Journal of Clinical Microbiology
Angela OatesAndrew J McBain

Abstract

Wound debridement samples and contralateral (healthy) skin swabs acquired from 26 patients attending a specialist foot clinic were analyzed by differential isolation and eubacterium-specific PCR-denaturing gradient gel electrophoresis (DGGE) in conjunction with DNA sequencing. Thirteen of 26 wounds harbored pathogens according to culture analyses, with Staphylococcus aureus being the most common (13/13). Candida (1/13), pseudomonas (1/13), and streptococcus (7/13) were less prevalent. Contralateral skin was associated with comparatively low densities of bacteria, and overt pathogens were not detected. According to DGGE analyses, all wounds contained significantly greater eubacterial diversity than contralateral skin (P < 0.05), although no significant difference in total eubacterial diversity was detected between wounds from which known pathogens had been isolated and those that were putatively uninfected. DGGE amplicons with homology to Staphylococcus sp. (8/13) and S. aureus (2/13) were detected in putatively infected wound samples, while Staphylococcus sp. amplicons were detected in 11/13 noninfected wounds; S. aureus was not detected in these samples. While a majority of skin-derived DGGE consortial fingerprints could be di...Continue Reading

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Citations

Dec 9, 2015·Journal of Diabetes and Its Complications·Alejandra Bravo-MolinaJosé Hernández-Quero
Feb 9, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rachel CromptonMatthew J Hardman
Nov 26, 2013·Diagnostic Microbiology and Infectious Disease·Jinhong XiaoCuiming Zhu
May 20, 2014·Journal of Diabetes Research·Angela OatesAndrew J McBain
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Jan 8, 2015·Advances in Wound Care·Jean-Philippe LavigneBenjamin A Lipsky
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Oct 18, 2020·Microorganisms·Cassandra PougetJean-Philippe Lavigne
Dec 29, 2020·Diabetes Research and Clinical Practice·Shili ZhangMengchen Zou

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