Molecular and cytogenetic analysis of the heterochromatin-euchromatin junction region of the Drosophila melanogaster X chromosome using cloned DNA sequences.

Genetics
M YamamotoG L Miklos

Abstract

We have used three cloned DNA sequences consisting of (1) part of the suppressor of forked transcription unit, (2) a cloned 359-bp satellite, and (3), a type I ribosomal insertion, to examine the structure of the base of the X chromosome of Drosophila melanogaster where different chromatin types are found in juxtaposition. A DNA probe from the suppressor of forked locus hybridizes exclusively to the very proximal polytenized part of division 20, which forms part of the beta-heterochromatin of the chromocenter. The cloned 359-bp satellite sequence, which derives from the proximal mitotic heterochromatin between the centromere and the ribosomal genes, hybridizes to the under replicated alpha-heterochromatin of the chromocenter. The type I insertion sequence, which has major locations in the ribosomal genes and in the distal mitotic heterochromatin of the X chromosome, hybridizes as expected to the nucleolus but does not hybridize to the beta-heterochromatic division 20 of the polytene X chromosome. Our molecular data reveal that the suppressor of forked locus, which on cytogenetic grounds is the most proximal ordinary gene on the X chromosome, is very close to the junction of the polytenized and non-polytenized region of the X ch...Continue Reading

Citations

Oct 7, 2003·Annual Review of Genomics and Human Genetics·Susan E Celniker, Gerald M Rubin
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·G L MiklosR Maleszka
Oct 1, 1992·Trends in Genetics : TIG·S M Williams, L G Robbins
Apr 1, 1994·Current Opinion in Genetics & Development·G H Karpen
Jun 3, 1999·Gene·K D CurtinR J Wyman
Mar 25, 2000·Science·M D AdamsJ C Venter
May 21, 2010·Experimental Animals·Masa-Toshi Yamamoto

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