Molecular and descriptive epidemiology of intestinal protozoan parasites of children and their pets in Cauca, Colombia: a cross-sectional study

BMC Infectious Diseases
Ximena VillamizarJuan D Ramírez

Abstract

Parasitic infections, particularly those caused by protozoa, represent a considerable public health problem in developing countries. Blastocystis, Giardia duodenalis, Cryptosporidium spp. and the Entamoeba complex (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii) are the most common etiological causes of intestinal parasitic infections. We carried out a descriptive cross-sectional study in school-age children attending a daycare institution in commune eight of Popayán, Cauca (Southwest Colombia). A total of 266 fecal samples were collected (258 from children and eight from pets). Blastocystis, G. duodenalis, Cryptosporidium spp. and the Entamoeba complex were identified by microscopy, quantitative real-time PCR (qPCR) and conventional PCR. The concordance of qPCR and microscopy was assessed using the Kappa index. Molecular characterization was conducted to identify Blastocystis subtypes (18S), G. duodenalis assemblages (tpi and gdh) and Cryptosporidium species/subtypes (18S and GP60). Potential associations between intestinal parasitism and sociodemographic factors were examined using bivariate analyses. A total of 258 fecal samples from children were analyzed by microscopy and 255 samples were analyzed by qPC...Continue Reading

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Citations

Jul 1, 2020·Parasitology Research·Ana Luz Galvan-DiazGisela Garcia-Montoya
Nov 10, 2020·Heliyon·Adriana HigueraJuan David Ramírez
Oct 14, 2020·Research in Veterinary Science·Josephine S Y HublinMonica Santin
Dec 3, 2020·Tropical Medicine and Infectious Disease·Kasandra Ascuña-DurandJorge Ballón-Echegaray
Mar 9, 2021·Veterinary Parasitology : Regional Studies and Reports·Paula C HernándezJenny Jovana Chaparro-Gutiérrez
Jun 2, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Renata Coltro BezagioMônica Lúcia Gomes
Jun 29, 2021·Parasite : Journal De La Société Française De Parasitologie·Huan TangYe Cao

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Methods Mentioned

BETA
PCR
genotyping
electrophoresis

Software Mentioned

STATA
BLAST
MEGA

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