Molecular and Electrophysiological Characterization of GABAergic Interneurons Expressing the Transcription Factor COUP-TFII in the Adult Human Temporal Cortex

Cerebral Cortex
Csaba VargaP Somogyi

Abstract

Transcription factors contribute to the differentiation of cortical neurons, orchestrate specific interneuronal circuits, and define synaptic relationships. We have investigated neurons expressing chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), which plays a role in the migration of GABAergic neurons. Whole-cell, patch-clamp recording in vitro combined with colocalization of molecular cell markers in the adult cortex differentiates distinct interneurons. The majority of strongly COUP-TFII-expressing neurons were in layers I-III. Most calretinin (CR) and/or cholecystokinin- (CCK) and/or reelin-positive interneurons were also COUP-TFII-positive. CR-, CCK-, or reelin-positive neurons formed 80%, 20%, or 17% of COUP-TFII-positive interneurons, respectively. About half of COUP-TFII-/CCK-positive interneurons were CR-positive, a quarter of them reelin-positive, but none expressed both. Interneurons positive for COUP-TFII fired irregular, accommodating and adapting trains of action potentials (APs) and innervated mostly small dendritic shafts and rarely spines or somata. Paired recording showed that a calretinin-/COUP-TFII-positive interneuron elicited inhibitory postsynaptic potentials (IPSPs) in a reciprocal...Continue Reading

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Citations

Jul 1, 2017·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Vincenzo Di LazzaroMarco Capogna
Jan 30, 2020·Journal of Neurophysiology·Kevin LeeJohanna M Montgomery
Jul 17, 2018·Frontiers in Cellular Neuroscience·Guy EyalIdan Segev
Jul 25, 2018·Current Protocols in Bioinformatics·Selim Kalayci, Zeynep H Gümüş
Oct 12, 2017·Frontiers in Neuroanatomy·Audrey Mercer, Alex M Thomson
Nov 21, 2020·Brain Communications·Thomas I-H ParkMichael Dragunow
Nov 17, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Helen C BarronCassandra Sampaio-Baptista

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