Molecular and expression analysis of an interferon-gamma-inducible guanylate-binding protein from rainbow trout (Oncorhynchus mykiss)

Developmental and Comparative Immunology
Børre RobertsenJo-Ann Leong

Abstract

Guanylate-binding proteins (GBPs) are some of the most abundant proteins accumulating in mammalian cells in response to interferon-gamma (IFN-gamma). GBPs have been suggested to function in antiviral activity, macrophage activation, fibroblast proliferation and inhibition of endothelial cell proliferation and invasiveness. Here we confirm that IFN-gamma-inducible GBP also exist in fish. A 2 kb GBP cDNA was cloned from head kidney of rainbow trout treated with an IFN-inducing compound. The open reading frame predicts a 635 amino acid protein (rbtGBP) of 72.7 kDa possessing a tripartite GTP binding motif and a secondary structure similar to human GBP1. Like most mammalian GBPs, rbtGBP possesses an isoprenylation motif at the C-terminal end. The overall amino acid sequence identity between rbtGBP and mammalian GBPs is only 41-47%, however. The rainbow trout macrophage cell line RTS11 showed a dose-dependent increase in rbtGBP transcripts in response to IFN-gamma after 6h of stimulation, with rbtGBP being undetectable in non-treated RTS11 cells. Moreover, polyinosinic polycytidylic acid (poly I:C) induced increased GBP transcript levels in RTS11 and RTG2 cells after 4-6 h of stimulation, and in head kidney and liver of live fish af...Continue Reading

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Citations

Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
Jul 26, 2011·Developmental and Comparative Immunology·Jun Zou, Christopher J Secombes
Jan 5, 2010·Developmental and Comparative Immunology·Azucena López-MuñozVictoriano Mulero
Sep 17, 2008·Developmental and Comparative Immunology·Linda D RhodesS Ellen Demlow
Aug 3, 2021·Aquaculture International : Journal of the European Aquaculture Society·Sweta DasThangaraj Raja Swaminathan
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Sascha Martens, Jonathan Howard

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