Molecular and functional characterization of the Gulf toadfish serotonin transporter SLC6A4

The Journal of Experimental Biology
Molly H B Amador, M Danielle McDonald

Abstract

The serotonin transporter (SERT) functions in the uptake of the neurotransmitter serotonin (5-HT) from the extracellular milieu and is the molecular target of the selective serotonin re-uptake inhibitors (SSRIs), a common group of anti-depressants. The current study comprehensively assesses the sequence, tissue distribution, transport kinetics and physiological function of a teleost SERT. The 2022 bp toadfish SERT sequence encodes a protein of 673 amino acids, which shows 83% similarity to zebrafish SERT and groups with SERT of other teleosts in phylogenetic analysis. SERT mRNA is ubiquitous in tissues and is expressed at high levels in the heart and, within the brain, in the cerebellum. SERT cRNA expressed in Xenopus laevis oocytes demonstrates a Km value of 2.08±0.45 μmol l-1, similar to previously reported Km values for zebrafish and human SERT. Acute systemic blockade of SERT by intraperitoneal administration of the SSRI fluoxetine (FLX) produces a dose-dependent increase in plasma 5-HT, indicating effective inhibition of 5-HT uptake from the circulation. As teleosts lack platelets, which are important 5-HT sequestration sites in mammals, the FLX-induced increase in plasma 5-HT suggests that toadfish tissues may normally be...Continue Reading

References

Jan 1, 1991·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Y Mori, M Ando
Jan 1, 1990·Annals of the New York Academy of Sciences·R W Fuller, D T Wong
Jan 1, 1984·Journal of Neuroscience Research·C Beas-ZarateA Feria-Velasco
Jan 1, 1983·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·D Piomelli, B Tota
Feb 1, 1995·Journal of Autonomic Pharmacology·C S VenugopalanK M Kleinow
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S RamamoorthyR D Blakely
May 1, 1995·The American Journal of Physiology·L SundinC O Löfman
Jan 1, 1993·Annual Review of Neuroscience·S G Amara, M J Kuhar
Apr 1, 1994·Neuron·S MagerH A Lester
Jan 1, 1996·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·J J JanvierP Soulier
Dec 31, 1996·Brain Research. Molecular Brain Research·A S ChangR D Blakely
Jun 18, 1998·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·D MarazzitiG B Cassano
Nov 3, 1998·Journal of Neurochemistry·N Nelson
Jul 17, 1999·Brain Research. Molecular Brain Research·O V MortensenO Wiborg
Jul 20, 2000·The Journal of Experimental Zoology·L Sundin, G E Nilsson
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S Nilsson, L Sundin
Dec 18, 2001·The Journal of Comparative Neurology·J Kaslin, P Panula
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 28, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·D PellegrinoB Tota
Mar 15, 2003·Progress in Neurobiology·Andrew H Bass, Jessica R McKibben
Apr 15, 2003·Toxicology Letters·Bryan W BrooksThomas W La Point
Apr 2, 2004·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Tal BarkanMoshe Rehavi
May 29, 2004·Journal of Cardiovascular Pharmacology·Wei NiStephanie W Watts
Nov 24, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Randal K Buddington, Ashild Krogdahl
Feb 22, 2005·Environmental Toxicology and Chemistry·Bryan W BrooksRussell J Lewis
Jul 26, 2005·Cellular and Molecular Life Sciences : CMLS·E Maurer-Spurej
Aug 9, 2005·Physiological and Biochemical Zoology : PBZ·Katherine A SlomanPatrick J Walsh
Feb 25, 2006·The Tohoku Journal of Experimental Medicine·Yuhong WangKomei Shirabe

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Citations

Oct 12, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Molly H B Amador, M Danielle McDonald
Jul 19, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Maria C CartolanoM Danielle McDonald

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