Molecular and functional characterization of grass carp squint/nodal-related 1: a potential regulator of activin signaling in teleost pituitary cells

Domestic Animal Endocrinology
T ZhaoHong Zhou

Abstract

Nodal, a member of the transforming growth factor-β superfamily, plays important roles in embryogenesis in vertebrates, including fish. However, the functional characterization of the fish nodal-related gene in nonembryonic cells is still unclear. In teleost, three nodal-related genes, nodal-related (ndr)1/squint, ndr2/cyclops, and ndr3/southpaw have been reported. In this study, a full-length cDNA for grass carp squint (gcSqt) was cloned, and its transcript was detected in the selected organs, including pituitary, brain, heart, head kidney, kidney, spleen, and gonad. To further define its functional role, recombinant grass carp squint (rgcSQT) was produced in Escherichia coli in a homodimer form. Furthermore, we examined the effects of rgcSQT on activin and its receptor gene expression with the use of grass carp pituitary cell as a model. Results showed that rgcSQT stimulated the mRNA expression of activin βA and βB subunit, as well as activin receptor ActRIB and ActRIIB. These findings not only contribute to the understanding of nonembryonic functions of nodal gene in fish, but they also provide new insight into the regulation of activin signaling in vertebrates.

References

Mar 1, 1995·Electrophoresis·L VuillardT Rabilloud
Oct 12, 2000·The Journal of Biological Chemistry·A KumarM R Kuehn
Mar 20, 2004·Trends in Cardiovascular Medicine·Nadira AhmadMichael Rebagliati
Sep 29, 2004·Molecular and Cellular Endocrinology·Louise M BilezikjianWylie W Vale
Nov 9, 2004·The Journal of Clinical Endocrinology and Metabolism·Guoxiong XuChun Peng
Dec 16, 2005·Nature·Aniket V GoreKaruna Sampath
Jul 13, 2006·Gene Expression Patterns : GEP·Daniele SoroldoniThomas Czerny
Feb 9, 2007·Development·Michael M Shen
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Hongmei Wang, Benjamin K Tsang
Nov 10, 2007·Development Genes and Evolution·Xiang Fan, Scott T Dougan
Nov 27, 2007·Developmental and Comparative Immunology·George HaddadMiodrag Belosevic
Dec 15, 2010·Nucleic Acids Research·Patrick C GilliganKaruna Sampath

❮ Previous
Next ❯

Citations

Feb 13, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Mara Lucia GravinattiFábio Gregori

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.