Molecular and functional characterization of novel fructosyltransferases and invertases from Agave tequilana.

PloS One
Celso Cortés-RomeroJune Simpson

Abstract

Fructans are the main storage polysaccharides found in Agave species. The synthesis of these complex carbohydrates relies on the activities of specific fructosyltransferase enzymes closely related to the hydrolytic invertases. Analysis of Agave tequilana transcriptome data led to the identification of ESTs encoding putative fructosyltransferases and invertases. Based on sequence alignments and structure/function relationships, two different genes were predicted to encode 1-SST and 6G-FFT type fructosyltransferases, in addition, 4 genes encoding putative cell wall invertases and 4 genes encoding putative vacuolar invertases were also identified. Probable functions for each gene, were assigned based on conserved amino acid sequences and confirmed for 2 fructosyltransferases and one invertase by analyzing the enzymatic activity of recombinant Agave protein s expressed and purified from Pichia pastoris. The genome organization of the fructosyltransferase/invertase genes, for which the corresponding cDNA contained the complete open reading frame, was found to be well conserved since all genes were shown to carry a 9 bp mini-exon and all showed a similar structure of 8 exons/7 introns with the exception of a cell wall invertase gene ...Continue Reading

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Citations

Aug 25, 2015·Frontiers in Plant Science·Emmanuel Avila de DiosJune Simpson
Jun 15, 2020·Journal of the Science of Food and Agriculture·Ma de Lourdes Pérez-ZavalaJosé E Barboza-Corona
Apr 9, 2020·Frontiers in Plant Science·Arely V Pérez-López, June Simpson

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Datasets Mentioned

BETA
EU026119
DQ535031

Methods Mentioned

BETA
glycosylation
PCR
Protein Assay

Software Mentioned

Signal P
SignalP
Clustal X
DNAstar
7500
BLAST
Agave
MEGA
EditSeq

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