Molecular and Functional Diversity of Crustin-Like Genes in the Shrimp Litopenaeus vannamei.

Marine Drugs
Shihao LiFuhua Li

Abstract

Crustins are crustacean cationic cysteine-rich antimicrobial peptides that contain one or two whey acidic protein (WAP) domain(s) at the carboxyl terminus and mainly show antimicrobial and/or proteinase inhibitory activities. Here, we performed genome and transcriptome screening and identified 34 full-length crustin-like encoding genes in Litopenaeus vannamei. Multiple sequence analysis of the deduced mature peptides revealed that these putative crustins included 10 type Ia, two type Ib, one type Ic, 11 type IIa, three type IIb, four type III, one type IV, one type VI, and one type VII. These putative crustins were clustered into different groups. Phylogenetic analysis, considering their domain composition, showed that different types of crustin-like genes in crustaceans might be originated from the WAP core region, along with sequence insertion, duplication, deletion, and amino acid substitution. Tissue distribution analysis suggested that most crustin-like genes were mainly detected in immune-related tissues while several crustin-like genes exhibited tissue-specific expression patterns. Quantitative PCR analysis on 15 selected crustin-like genes showed that most of them were apparently upregulated after Vibrio parahaemolyticu...Continue Reading

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Datasets Mentioned

BETA
MT375591
MT375592
MT375593
MT375594
ACQ66004

Methods Mentioned

BETA
environmental stresses
RNA-seq
electrophoresis
PCR

Software Mentioned

ORFfinder
Morpheus
Primer
ClustalW2
ProtParam
Trinity
MEGA5
SPSS

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