Molecular and functional effects of organismal ageing on smooth muscle cells derived from bone marrow mesenchymal stem cells.

Cardiovascular Research
Juhee HanStelios T Andreadis

Abstract

Bone marrow-derived smooth muscle cells (BM-SMCs) have high potential as an autologous cell source of vascular progenitors but normal cell function and turnover frequency may decline with age. In this study we set out to study the effects of organismal ageing on the molecular and functional properties of BM-SMCs. To address this issue, we employed a smooth muscle alpha-actin promoter (alphaSMA) driving expression of enhanced green fluorescence protein (EGFP) to isolate SMCs from bone marrow of neonatal (nBM-SMCs) or adult (aBM-SMCs) sheep and examined their proliferation potential and contractility. Compared with nBM-SMCs, aBM-SMCs exhibited lower clonogenicity and proliferation potential that could be improved significantly by addition of basic fibroblast growth factor. Vascular constructs from aBM-SMCs showed reduced ability to generate force and contract fibrin hydrogels and this function could be partially restored by addition of transforming growth factor-beta1. They also exhibited lower receptor- and non-receptor-mediated vascular contractility and mechanical strength, which was comparable to that of tissue constructs prepared with vascular SMCs from neonatal umbilical veins. In agreement with the contractile properties a...Continue Reading

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Citations

Mar 7, 2012·Applied Biochemistry and Biotechnology·Wan Kamarul Zaman Wan SafwaniKien Hui Chua
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Feb 9, 2021·Advanced Functional Materials·Bita NasiriStelios T Andreadis

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