PMID: 3082627Mar 17, 1986

Molecular and functional properties of protein SS1 from small ribosomal subunits of Streptomyces aureofaciens

European Journal of Biochemistry
K MikulíkP Branny

Abstract

Small ribosomal subunits of gram-positive cells of Streptomyces aureofaciens contain an acidic protein designated SS1. Purified protein SS1 has the same mobility in sodium dodecyl sulfate/polyacrylamide gel as ribosomal protein S1 of Escherichia coli (apparent Mr 68 000). Protein SS1 was dissected under mild conditions with trypsin and generated fragments were compared with well-characterized fragments of protein S1. The protein SS1 contains a structure homologous with the C-terminal fragment of protein S1. The affinity of protein SS1 to poly(U) is virtually identical with that of E. coli protein S1. In contrast to protein S1, the addition of SS1 to partially S1-depleted ribosomes of E. coli had no stimulatory effect on poly(U)-directed synthesis of polyphenylalanine. At molar excess of SS1 over ribosomes, the protein had comparable inhibitory effect on polypeptide synthesis as had S1 of E. coli. Ribosomes of S. aureofaciens required about one order of magnitude higher concentration of poly(U) for maximum synthetic activity than did ribosomes of E. coli. The addition of proteins SS1 or S1 to ribosomes of S. aureofaciens had no stimulatory effect on translation of poly(U). Our data indicate that the high-molecular-mass acidic pr...Continue Reading

References

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Related Concepts

M1-S1 ribosomal protein
Ribosomal protein SS1, Streptomyces aureofaciens
Bacterial Proteins
Alkalescens-Dispar Group
Hydrolysis
Polyglobin
Immunochemistry
Poly U
Plasma Protein Binding Capacity
Ribosomal Proteins

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