Molecular and genetic characterization of a novel bacteriocin locus in Enterococcus avium isolates from infants.

Applied and Environmental Microbiology
Dagim Jirata BirriIngolf F Nes

Abstract

Enterococci are among the most common human intestinal lactic acid bacteria, and they are known to produce bacteriocins. In this study, fecal enterococci were isolated from infants and screened for bacteriocin production. Bacteriocin-producing Enterococcus avium isolates were obtained, and a new pediocin-like bacteriocin was purified and characterized. This bacteriocin, termed avicin A, was found to be produced by isolates from two healthy infants. It was purified to homogeneity from culture supernatant by ion-exchange and reversed-phase chromatography, and part of its amino acid sequence was obtained. The sequence of a 7-kb DNA fragment of a bacteriocin locus was determined by PCR and DNA sequencing. The bacteriocin locus was organized into four operon-like structures consisting of (i) the structural genes encoding avicin A and its immunity protein, (ii) a divergicin-like bacteriocin (avicin B) gene, (iii) an ABC bacteriocin transporter gene and two regulatory genes (histamine protein kinase- and response regulator-encoding genes), and (iv) induction peptide pheromone- and transport accessory protein-encoding genes. It was shown that the production of avicin A was regulated by the peptide pheromone-inducible regulatory system....Continue Reading

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Citations

Jan 22, 2013·Applied and Environmental Microbiology·Shun IwataniKenji Sonomoto
Dec 12, 2012·International Journal of Molecular Sciences·Yanhua CuiHongyu Cui
Oct 31, 2012·Cellular and Molecular Life Sciences : CMLS·Riadh HammamiIsmail Fliss
Nov 21, 2015·Journal of Agricultural and Food Chemistry·Sara ArbuluPablo E Hernández
Mar 4, 2015·Computational Biology and Chemistry·Neetigyata Pratap SinghReema Gabrani
Jul 1, 2017·Nihon saikingaku zasshi. Japanese journal of bacteriology·Yasuyoshi Ike
Mar 22, 2018·Applied Microbiology and Biotechnology·Sen Zheng, Kenji Sonomoto
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Charles Y WangSuzanne Dawid
Sep 28, 2021·Frontiers in Microbiology·Xinyi GuJian Kong

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