Abstract
Mutations of the p53 gene appear to be one of the most common abnormalities in human cancer. Although many studies have been published about p53 alterations in breast cancer, data on molecular biological detection of p53 mutations in in situ lesions are still rare, and the implications for breast cancerogenesis are unclear. Tissue samples from 83 patients with different stages of breast cancer and from 13 patients with benign breast lesions were screened for p53 gene mutations by polymerase chain reaction (PCR) followed by temperature-gradient gel electrophoresis (TGGE). p53 protein accumulation was analysed by immunohistochemistry (IHC). Samples were gained from fresh-frozen tissue, scrapings, or paraffin embedded tissue. Additionally, 23 pairs of primary tumours and corresponding lymph nodes were examined. p53 gene aberrations were found in 55.7% of the infiltrating carcinomas, in 31.5% of the ductal carcinomas in situ (DCIS) and in one atypical ductal hyperplasia. A positive correlation was seen with high-grade tumours and with comedo. There was no statistically significant relationship with respect to age, menopausal status, tumour size, hormone receptor status or lymphatic invasion. Concordance between TGGE and IHC was see...Continue Reading
Citations
May 2, 2000·Diagnostic Cytopathology·S GudlaugsdottirJ E Eyfjord
Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·R KandelT Rohan
Dec 21, 2000·Cancer Letters·Q ZhouP S Steeg
May 3, 2001·Journal of the National Cancer Institute·S J DoneI L Andrulis
Feb 23, 2012·Cellular Oncology (Dordrecht)·Cigdem ErcanPatrick W B Derksen
Jul 16, 2009·Journal of Cellular and Molecular Medicine·Pavel RossnerRegina M Santella
Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·A SidoniE Bucciarelli
Aug 27, 2011·Journal of Oncology·Geoffrey C KabatThomas E Rohan
Jun 2, 2016·Human Pathology·Xi WangDavid G Hicks
Jul 13, 2004·Cancer Letters·Phouthone KeohavongMona F Melhem
Aug 26, 2016·Breast Cancer : Basic and Clinical Research·David N Danforth
Jul 16, 2013·Journal of Clinical Laboratory Analysis·An HeYing-Song Wu
Feb 9, 2013·Polish Journal of Veterinary Sciences·A Kycko, M Reichert
Sep 16, 2017·Breast Cancer Research and Treatment·David N Danforth
Jan 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicolaus KrögerThomas Löning
Dec 6, 2000·Gynecologic Oncology·C GoepelH Koelbl