PMID: 9428925Jan 16, 1998Paper

Molecular and immunohistochemical analysis of p53 mutations in scrapings and tissue from preinvasive and invasive breast cancer

Virchows Archiv : an International Journal of Pathology
B W LisboaT Löning

Abstract

Mutations of the p53 gene appear to be one of the most common abnormalities in human cancer. Although many studies have been published about p53 alterations in breast cancer, data on molecular biological detection of p53 mutations in in situ lesions are still rare, and the implications for breast cancerogenesis are unclear. Tissue samples from 83 patients with different stages of breast cancer and from 13 patients with benign breast lesions were screened for p53 gene mutations by polymerase chain reaction (PCR) followed by temperature-gradient gel electrophoresis (TGGE). p53 protein accumulation was analysed by immunohistochemistry (IHC). Samples were gained from fresh-frozen tissue, scrapings, or paraffin embedded tissue. Additionally, 23 pairs of primary tumours and corresponding lymph nodes were examined. p53 gene aberrations were found in 55.7% of the infiltrating carcinomas, in 31.5% of the ductal carcinomas in situ (DCIS) and in one atypical ductal hyperplasia. A positive correlation was seen with high-grade tumours and with comedo. There was no statistically significant relationship with respect to age, menopausal status, tumour size, hormone receptor status or lymphatic invasion. Concordance between TGGE and IHC was see...Continue Reading

Citations

Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·R KandelT Rohan
May 3, 2001·Journal of the National Cancer Institute·S J DoneI L Andrulis
Feb 23, 2012·Cellular Oncology (Dordrecht)·Cigdem ErcanPatrick W B Derksen
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Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·A SidoniE Bucciarelli
Aug 26, 2016·Breast Cancer : Basic and Clinical Research·David N Danforth
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Jan 7, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nicolaus KrögerThomas Löning
Dec 6, 2000·Gynecologic Oncology·C GoepelH Koelbl

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