Abstract
To identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, Vegf, Ngf, Ki67, Myh11, and Myh2; (2) expression of smooth and striated muscle proteins. MDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2 h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real-time RT-qPCR and immunohistochemistry were performed. MDSCs were identified only after 2 h of the procedure in the urethra. Myh11 gene was overexpressed in the Trauma group in relation to Control. Ki67 gene expression was increased in the MDSC group relative to Trauma and Control. Col1a1 and Col3a1 genes expression were increased in the MDSC group relative to Control. Ngf mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control. MDSCs migrated early to...Continue Reading
References
May 8, 2000·Neurourology and Urodynamics·M B ChancellorJ Huard
Aug 30, 2002·International Review of Neurobiology·Stuart C Apfel
Feb 26, 2003·International Urogynecology Journal and Pelvic Floor Dysfunction·J Y LeeM B Chancellor
Nov 20, 2003·Urology·Tracy W CannonMichael B Chancellor
Apr 10, 2004·Urology·Christopher J ChermanskyMichael B Chancellor
Aug 15, 2006·Urology·Dongdeuk KwonMichael B Chancellor
Apr 17, 2007·Molecular and Cellular Biochemistry·Akira Wagatsuma
Apr 19, 2008·International Urogynecology Journal and Pelvic Floor Dysfunction·Akira FurutaMichael B Chancellor
Jun 17, 2008·The Journal of Urology·Hadley M WoodMargot S Damaser
Sep 6, 2008·Nature Protocols·Burhan GharaibehJohnny Huard
Dec 19, 2008·American Journal of Physiology. Renal Physiology·Hui Q PanMargot S Damaser
Nov 3, 2009·Cytotherapy·Guiting LinChing-Shwun Lin
Mar 26, 2010·Tissue Engineering. Part B, Reviews·Jeffrey A BeamishRoger E Marchant
May 4, 2010·Journal of Korean Medical Science·Joa-Jin LimKyung-Jin Lee
May 25, 2010·Cell and Tissue Research·Xiaoyun WuXinling An
Nov 23, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y XuZ X Lin
Mar 18, 2011·Neurourology and Urodynamics·Jacques CorcosJacques Galipeau
Apr 27, 2011·Neurourology and Urodynamics·Peter OchodnickýMartin C Michel
Sep 24, 2011·Obstetrics and Gynecology International·Michelle CruzMargot Damaser
Oct 2, 2012·The Journal of Urology·Andrew T LenisHadley M Wood
Jul 23, 2013·Cell Transplantation·Charuspong DissarananMargot S Damaser
Jul 1, 2014·Urology·Matthias D HoferArun K Sharma
Apr 4, 2015·Yonsei Medical Journal·Jae Young ChoiTae Gyun Kwon