Molecular and immunological analysis of an ABC transporter complex required for cytochrome c biogenesis

Journal of Molecular Biology
B S GoldmanR G Kranz

Abstract

The helABC genes are predicted to encode an ATP-binding cassette (ABC) transporter necessary for heme export for ligation in bacterial cytochrome c biogenesis. The recent discoveries of homologs of the helB and helC genes in plant mitochondrial genomes suggest this is a highly conserved transporter in prokaryotes and some eukaryotes with the HelB and HelC proteins comprising the transmembrane components. Molecular genetic analysis in the Gram-negative bacterium Rhodobacter capsulatus was used to show that the helABC and helDX genes are part of an operon linked to the secDF genes. To facilitate analysis of this transporter, strains with non-polar deletions in each gene, epitope and reporter-tagged HelABCD proteins, and antisera specific to the HelA and HelX proteins were generated. We directly demonstrate that this transporter is present in the cytoplasmic membrane as an HelABCD complex. The HelB and HelC but not HelD proteins are necessary for the binding and stability of the HelA protein, the cytoplasmic subunit containing the ATP-binding region. In addition we show that the HelA protein co-immunoprecipitates with either the HelC or HelD proteins. Thus, the HelABCD heme export complex is distinguished by the presence of four m...Continue Reading

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Citations

Nov 10, 2011·Biochemistry·Brian San FranciscoRobert G Kranz
Aug 28, 1998·Molecular Microbiology·R KranzS Merchant
Jan 16, 2013·The Journal of Biological Chemistry·Andreia F VerissimoFevzi Daldal
Mar 11, 2008·Journal of Bacteriology·Cynthia L Richard-FogalRobert G Kranz
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Jul 6, 2010·Journal of Molecular Biology·Cynthia Richard-Fogal, Robert G Kranz

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