Molecular and immunological characterization of cytochrome c: a potential cross-reactive allergen in fungi and grasses

Allergy
V SharmaNaveen Arora

Abstract

Recombinant allergens are required for component-resolved diagnosis/therapy of allergic disorders. The study was aimed to express and characterize an allergenic protein from Curvularia lunata and study its cross-reactivity. A clone encoding a 12-kDa protein screened from the cDNA library of C. lunata was sequenced and expressed in pET22b+ vector. The purified protein was characterized by biophysical and immunological methods. The sequence of gene encoding a 12-kDa protein showed homology to cytochrome c. It was expressed in Escherichia coli yielding 0.5 mg protein/l culture and designated as Cur l 3. The absorption and circular dichroism spectrum of Cur l 3 were similar to horse cytochrome c and the protein reacted with cytochrome c antibody. ELISA with different fungal-positive patients' sera showed > or = 3 times specific IgE to Cur l 3 compared with healthy controls. Mice anti-Cur l 3 reacted with tropical and temperate grass extracts. Protein also reacted with grass-positive patients' sera. In vitro stimulation of peripheral blood mononuclear cells from C. lunata, fungi or grass-positive patients with it released significant levels of Th2 cytokines. In vivo testing of this protein in allergic patients showed marked positive...Continue Reading

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Citations

Jun 11, 2011·American Journal of Respiratory Cell and Molecular Biology·Vidhu SharmaNaveen Arora
Jul 17, 2019·Current Protein & Peptide Science·Lisha LiSong Guo Zheng
Jun 8, 2021·Molecular Immunology·Nicolas VisezPascal Poncet

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