PMID: 9523689Apr 2, 1998Paper

Molecular and immunological characterization of group V allergen isoforms from velvet grass pollen (Holcus lanatus)

European Journal of Biochemistry
G SchrammW M Becker

Abstract

Group V grass pollen allergens belong to the major grass pollen allergens causing reactions of type I allergy. cDNAs coding for two isoforms of the group V allergen of velvet grass pollen (Holcus lanatus), a widespread grass species, were isolated from a cDNA library by hybridization with a 5'-terminal reverse-transcribed PCR-derived cDNA probe. Amino acid sequences of the two isoforms, designated rHol l 5.01 and rHol l 5.02, revealed high similarity between them (78% identity) and to group V allergens and their isoforms found in other grass species. Recombinant Hol l 5 isoforms were expressed in Escherichia coli and purified as fusion proteins. To compare their immunological reactivities with group-V-specific monoclonal antibodies and patients' IgE, immunoblotting, ELISA and histamine release assay were performed. Interestingly, monoclonal antibody Bo9, specific for group Vb isoforms of timothy grass, bound only to isoform rHol l 5.01, not to rHol l 5.02. On the other side, IgE reactivities of patients' sera revealed no differences between the two isoforms when investigated by immunoblotting and only slight differences when investigated by ELISA. In histamine release assay both isoforms released comparable amounts of histamine...Continue Reading

Citations

Sep 13, 2001·Veterinary Immunology and Immunopathology·P B Hill, D J DeBoer
Sep 21, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C Suphioglu
Mar 17, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G SchrammW M Becker
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ronald van Ree
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·A R LorenzS Vieths

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