Molecular and kinetic characterization of histamine transport into adult rat cultured astrocytes

Neurochemistry International
Katja Perdan-PirkmajerMojca Krzan

Abstract

Astrocytes have a key role in the clearance and inactivation of histamine in the adult central nervous system, but transporters which mediate histamine uptake into astrocytes have not been fully characterized. We therefore investigated the kinetic and molecular characteristics of histamine uptake into cultured adult rat astrocytes. [(3)H]-histamine was taken up by astrocytes in a temperature-, time- and concentration-dependent manner and was inhibited up to 60-70% by 1mM ouabain or by substitution of NaCl with choline chloride. Specific [(3)H]-histamine uptake, determined as the difference between transport at 37 and 4°C, displayed saturation kinetics with the apparent Michaelis-Menten constant (K(m)) of 141 and 101μM and the apparent maximal uptake rate (V(max)) of 22.5 and 17.8pmol/min/mg protein, as estimated from the Woolf and the Eadie-Hofstee plots, respectively. Since our data suggested the presence of a carrier-operated histamine uptake system, we assessed the possible involvement of the organic cation transporters (OCT) 1, 2 and 3, which have been previously described to play a role in histamine transport in the central nervous system. Low level mRNA expression of all OCT isoforms was detected, but in contrast to rat b...Continue Reading

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Citations

Apr 4, 2013·Neurochemical Research·Katja Perdan-PirkmajerMojca Krzan
Aug 1, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Damijana M JuričMetoda Lipnik-Stangelj
Jul 9, 2017·Neurochemical Research·Eva-Maria Blumrich, Ralf Dringen

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